CyFlow CD16 Biotin (RUO)
| 品番 | AA614260 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD16 BIOTIN,3G8 | ||
| 包装単位 | 0.1 mg | ||
| 濃度 | 1 mg/ml | ||
| 推奨使用量 | - | ||
| 容量 | 0.1 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG1 Biotin (AQ807476) |
||
| 反応性|交差吸着 | Human | Non-Human Primates | レーザー | - |
| 抗原 | CD16, FcγRIIIA, CD16a | 最大蛍光波長 | - |
| クローン | 3G8 | 最大励起波長 | - |
| ホスト | Mouse | 標識/Format | Biotin |
| アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
| クローナリティ | monoclonal | アプリケーション | - |
Anti-Hu CD16 BIOTIN,3G8
特異性
The mouse monoclonal antibody 3G8 recognizes CD16 antigen, a low affinity receptor for aggregated IgG (FcγRIII antigen). CD16 exists in two different isoforms: CD16a (FcγRIIIA; 50-65 kDa; expressed on NK-cells, monocytes and macrophages) and CD16b (FcγRIIIB; 48 kDa; mainly expressed on neutrophils).
抗原情報
CD16 (FcγRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcγRIII is expressed in two forms - FcγRIIIA and FcγRIIIB. FcγRIIIA is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcεRI-γ subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcγRIIIA is associated, moreover, with FcεRI-β subunit. Besides IgG, FcγRIIIA can be triggered also by oligomeric IgE. FcγRIIIB is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
利用方法
The reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Working concentrations should be determined by the investigator.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Burt BM, Plitas G, Zhao Z, Bamboat ZM, Nguyen HM, Dupont B, DeMatteo RP: The lytic potential of human liver NK cells is restricted by their limited expression of inhibitory killer Ig‑like receptors. J·Immunol. 2009·Aug·1; 183(3):1789‑96. <·PMID:·19587011·>
• Choi EI, Wang R, Peterson L, Letvin NL, Reimann KA: Use of an anti‑CD16 antibody for in vivo depletion of natural killer cells in rhesus macaques. Immunology. 2008·Jun; 124(2):215‑22. <·PMID:·18201184·>
• Congy-Jolivet N, Bolzec A, Ternant D, Ohresser M, Watier H, Thibault G: Fc gamma RIIIa expression is not increased on natural killer cells expressing the Fc gamma RIIIa‑158V allotype. Cancer·Res. 2008·Feb·15; 68(4):976‑80. <·PMID:·18281470·>
• Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·>
• Komano Y, Nanki T, Hayashida K, Taniguchi K, Miyasaka N: Identification of a human peripheral blood monocyte subset that differentiates into osteoclasts. Arthritis·Res·Ther. 2006; 8(5):R152. <·PMID:·16987426·>
• Metes D, Ernst LK, Chambers WH, Sulica A, Herberman RB, Morel PA: Expression of functional CD32 molecules on human NK cells is determined by an allelic polymorphism of the FcgammaRIIC gene. Blood. 1998·Apr·1; 91(7):2369‑80. <·PMID:·9516136·>
• Wijngaarden S, van Roon JA, van de Winkel JG, Bijlsma JW, Lafeber FP: Down‑regulation of activating Fcgamma receptors on monocytes of patients with rheumatoid arthritis upon methotrexate treatment. Rheumatology·(Oxford). 2005·Jun; 44(6):729‑34. <·PMID:·15757966·>
• Zhu X, Hamann KJ, Muňoz NM, Rubio N, Mayer D, Herrnreiter A, Leff AR: Intracellular expression of Fc gamma RIII (CD16) and its mobilization by chemoattractants in human eosinophils. J·Immunol. 1998·Sep·1; 161(5):2574‑9. <·PMID:·9725258·>
• Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford·University·Press,·Oxford. 1989; 1‑1820. <·NLM·ID:·8914679·>