特異性

The mouse monoclonal antibody UCHL1 recognizes CD45R0, a 180 kDa low molecular weight isoform of the leukocyte common antigen (LCA). CD45R0 is expressed on a subset of memory/activated T cells and on cortical thymocytes.

抗原情報

CD45R0 is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45R0 is expressed e.g. on macrophages, CD8+ T cells, activated T cells and myeloma cells.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

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• Bijian K, Zhang L, Shen SH: Collagen‑mediated survival signaling is modulated by CD45 in Jurkat T cells. Mol·Immunol. 2007·Jul; 44(15):3682‑90. <·PMID:·17524482·>
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• Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·>
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• Merkenschlager M, Terry L, Edwards R, Beverley PC: Limiting dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T cell memory formation. Eur·J·Immunol. 1988·Nov; 18(11):1653‑61. <·PMID:·2974420·>
• Norton AJ, Ramsay AD, Smith SH, Beverley PC, Isaacson PG: Monoclonal antibody (UCHL{01} that recognises normal and neoplastic T cells in routinely fixed tissues. J·Clin·Pathol. 1986·Apr; 39(4):399‐405. <·PMID:·2422219·>
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• Terry LA, Brown MH, Beverley PC: The monoclonal antibody, UCHL1, recognizes a 180,000 MW component of the human leucocyte‑common antigen, CD45. Immunology. 1988·Jun; 64(2):331‑6. <·PMID:·2455685·>
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