CyFlow Perforin PE (RUO)
| 品番 | AC676621 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu PERFORIN PE,dG9 | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 推奨使用量 | - | ||
| 容量 | 1 ml | ||
| 関連製品 (アイソタイプコントロール) |
- | ||
| 反応性|交差吸着 | Human | Cow | レーザー | Blue, Green, Yellow |
| 抗原 | Perforin | 最大蛍光波長 | 576 nm |
| クローン | dG9 | 最大励起波長 | 496 nm, 565 nm |
| ホスト | Mouse | 標識/Format | PE |
| アイソタイプ | IgG2b | 研究分野 | Apoptosis |
| クローナリティ | monoclonal | アプリケーション | - |
Anti-Hu PERFORIN PE,dG9
特異性
The mouse monoclonal antibody dG9 (δG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells.
抗原情報
Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Hersperger AR1, Makedonas G, Betts MR: Flow cytometric detection of perforin upregulation in human CD8 T cells. Cytometry·A. 2008·Nov; 73(11):1050‑7. <·PMID:·18615597·>
• Qiu Y, Chen J, Liao H, Zhang Y, Wang H, Li S, Luo Y, Fang D, Li G, Zhou B, Shen L, Chen CY, Huang D, Cai J, Cao K, Jiang L, Zeng G, Chen ZW: Tim‑3‑expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti‑TB effector functions. PLoS·Pathog. 2012; 8(11):e1002984. <·PMID:·23144609·>
• Takeuchi M, Inoue T, Otani T, Yamasaki F, Nakamura S, Kibata M: Cell‑in‑cell structures formed between human cancer cell lines and the cytotoxic regulatory T‑cell line HOZOT. J·Mol·Cell·Biol. 2010·Jun; 2(3):139‑51. <·PMID:·20348186·>
• Vargas-Inchaustegui DA, Demberg T, Robert-Guroff M: A CD8α(‑) subpopulation of macaque circulatory natural killer cells can mediate both antibody‑dependent and antibody‑independent cytotoxic activities. Immunology. 2011·Nov; 134(3):326‑40. <·PMID:·21978002·>