CyFlow HLA-Class I PE (RUO)
| 品番 | AF666095 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu HLA-ClAss I PE,W6/32 | ||
| 包装単位 | 0.1 mg | ||
| 濃度 | 0.1 mg/ml | ||
| 推奨使用量 | - | ||
| 容量 | 1 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG2a PE (BY503796) |
||
| 反応性|交差吸着 | Human | Non-Human Primates | Cow | Cat | レーザー | Blue, Green, Yellow |
| 抗原 | HLA-Class I | 最大蛍光波長 | 576 nm |
| クローン | W6/32 | 最大励起波長 | 496 nm, 565 nm |
| ホスト | Mouse | 標識/Format | PE |
| アイソタイプ | IgG2a | 研究分野 | MHC |
| クローナリティ | monoclonal | アプリケーション | - |
Anti-Hu HLA-ClAss I PE,W6/32
特異性
The mouse monoclonal antibody W6/32 recognises MHC Class I molecules (MHC Class Ia) that are expressed on the surface of all human nucleated cell types. The antibody W6/32 is a valuable reagent for analyzing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders. The W6/32 is also suitable as a positive control for HLA tissue typing and crossmatching.
抗原情報
HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant β2-microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumor defense. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
利用方法
The reagent is designed for Flow Cytometry analysis. Suggested working usage is 2 µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Barnstable CJ, Bodmer WF, Brown G, Galfre G, Milstein C, Williams AF, Ziegler A: Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens ‑ new tools for genetic analysis. Cell. 1978; 14:9‑20. <·PMID:·667938·>
• Brodsky FM, Parham P: Evolution of HLA antigenic determinants: species cross reactions of monoclonal antibodies. Immunogenetics. 1982; 15:151‑166. <·PMID:·6174442·>
• Jacobsen CN, Aasted B, Broe MK, Petersen JL: Reactivities of 20 anti‑human monclonal antibodies with leucocytes from ten different animal species. Vet·Immunopathol. 1993; 39:461‑466. <·PMID:·8116221·>
• Kievits F, Ivanyi P: Monomorphic anti‑HLA monoclonal antibody (W6/32) recognizes polymorphic H‑2 heavy‑chain determinants exposed by association with bovine or human but not murine beta 2‑microglobulin.. Hum·Immunol. 1987·Oct; 20(2):115‑26. <·PMID:·3316135·>
• Ladasky JJ, Shum BP, Canavez F, Seuanez HN, Parham P: Residue 3 of beta2‑microglobulin affects binding of class I MHC molecules by the W6/32 antibody. Immunogenetics. 1999·Apr; 49(4):312‑20. <·PMID:·10079295·>
• Le Discorde M, Moreau P, Sabatier P, Legeais JM, Carosella ED: Expression of HLA‑G in human cornea, an immune‑privileged tissue. Hum·Immunol. 2003·Nov; 64(11):1039‑44. <·PMID:·14602233·>
• Neefjes JJ, Hensen EJ, de Kroon TI, Ploegh HL: A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes. Immunogenetics. 1986; 23:341‑347. <·PMID:·3458670·>
• Shields MJ, Ribaudo RK: Mapping of the monoclonal antibody W6/32: sensitivity to the amino terminus of beta‑microglobulin. Tissue·Antigens·. 1998·May; 51(5):567‑70. <·PMID:·9672158·>
• Stern PL, Beresford N, Friedman CI, Stevens VC, Risk JM, Johnson PM: Class I‑like MHC molecules expressed by baboon placental synctiotrophoblast. J·Immunol. 1987; 138(4):1088‑1091. <·PMID:·3543121·>
• Tran TM, Ivanyi P, Hilgert I, Brdicka T, Pla M, Breur B, Flieger M, Ivaskova E, Horejsi V: The epitope recognized by pan‑HLA class I‑reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA‑B27/beta2‑microglobulin complex. Immunogenetics. 2001·Aug; 53(6):440‑6. <·PMID:·11685454·>