CyFlow TNF-alpha APC (RUO)
| 品番 | AF800796 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu TNF-ALPHA APC,MAb11 | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 推奨使用量 | - | ||
| 容量 | 1 ml | ||
| 関連製品 (アイソタイプコントロール) |
- | ||
| 反応性|交差吸着 | Human | Non-Human Primates | Pig | レーザー | Red |
| 抗原 | TNF-α | 最大蛍光波長 | 660 nm |
| クローン | MAb11 | 最大励起波長 | 650 nm |
| ホスト | Mouse | 標識/Format | APC |
| アイソタイプ | IgG1 | 研究分野 | Signaling |
| クローナリティ | monoclonal | アプリケーション | - |
Anti-Hu TNF-ALPHA APC,MAb11
特異性
The mouse monoclonal antibody MAb11 recognizes human 17-26 kDa cytokine TNF α (tumor necrosis factor α).
抗原情報
TNF α is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF α easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Attarbaschi T, Willheim M, Ramharter M, Hofmann A, Wahl K, Winkler H, Graninger W, Winkler S: T cell cytokine profile during primary Epstein‑Barr virus infection (infectious mononucleosis). Eur·Cytokine·Netw. 2003·Jan-Mar; 14(1):34‑9. <·PMID:·12799212·>
• Cesaro-Tadic S, Dernick G, Juncker D, Buurman G, Kropshofer H, Michel B, Fattinger C, Delamarche E: High‑sensitivity miniaturized immunoassays for tumor necrosis factor alpha using microfluidic systems. Lab·Chip. 2004·Dec; 4(6):563‑9. <·PMID:·15570366·>
• Visser J, Graffelman W, Blauw B, Haspels I, Lentjes E, de Kloet ER, Nagelkerken L: LPS‑induced IL‑10 production in whole blood cultures from chronic fatigue syndrome patients is increased but supersensitive to inhibition by dexamethasone. J·Neuroimmunol. 2001·Oct·1; 119(2):343‑9. <·PMID:·11585638·>
• Wahlström J, Katchar K, Wigzell H, Olerup O, Eklund A, Grunewald J: Analysis of intracellular cytokines in CD4+ and CD8+ lung and blood T cells in sarcoidosis. Am·J·Respir·Crit·Care·Med. 2001·Jan; 163(1):115‑21. <·PMID:·11208635·>
• Yan SR, Qing G, Byers DM, Stadnyk AW, Al-Hertani W, Bortolussi R: Role of MyD88 in diminished tumor necrosis factor alpha production by newborn mononuclear cells in response to lipopolysaccharide. Infect·Immun. 2004·Mar; 72(3):1223‑9. <·PMID:·14977922·>