CyFlow CD31 Biotin (RUO)
| 品番 | AG002104 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD31 BIOTIN,MEM-05 | ||
| 包装単位 | 0.1 mg | ||
| 濃度 | 1 mg/ml | ||
| 推奨使用量 | - | ||
| 容量 | 0.1 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG1 Biotin (AQ807476) |
||
| 反応性|交差吸着 | Human | レーザー | - |
| 抗原 | CD31, PECAM-1, endoCAM, PECA1 | 最大蛍光波長 | - |
| クローン | MEM-05 | 最大励起波長 | - |
| ホスト | Mouse | 標識/Format | Biotin |
| アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
| クローナリティ | monoclonal | アプリケーション | - |
Anti-Hu CD31 BIOTIN,MEM-05
特異性
The mouse monoclonal antibody MEM-05 recognizes CD31 antigen, a 130-140 kDa type I transmembrane glycoprotein expressed on monocytes, platelets, granulocytes, endothelial cells and stem cells of the myeloid lineage.
抗原情報
CD31 (PECAM-1; platelet endothelial cell adhesion molecule-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.
利用方法
The reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 2 µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Cârţână T, Săftoiu A, Gruionu LG, Gheonea DI, Pirici D, Georgescu CV, Ciocâlteu A, Gruionu G: Confocal laser endomicroscopy for the morphometric evaluation of microvessels in human colorectal cancer using targeted anti‑CD31 antibodies. PLoS·One. 2012; 7(12):e52815. <·PMID:·23285192·>
• Jackson DE: The unfolding tale of PECAM‑1. FEBS·Lett. 2003·Apr·10; 540(1‑3):41821. <·PMID:·12681475·>
• Newman DK, Hamilton C, Newman PJ: Inhibition of antigen‑receptor signaling by Platelet Endothelial Cell Adhesion Molecule‑1 (CD31 requires functional ITIMs, SHP‑2, and p56(lck). Blood. 2001·Apr·15; 97(8):2351‑7. <·PMID:·11290597·>
• Prager E, Staffler G, Majdic O, Saemann M, Godar S, Zlabinger G, Stockinger H: Induction of hyporesponsiveness and impaired T lymphocyte activation by the CD31 receptor:ligand pathway in T cells. J·Immunol. 2001·Feb·15; 166(4):2364‑71. <·PMID:·11160294·>
• Wee JL, Jackson DE: The Ig‑ITIM superfamily member PECAM‑1 regulates the "outside‑in" signaling properties of integrin alpha(IIb)beta3 in platelets. Blood. 2005·Dec·1; 106(12):3816‑23. <·PMID:·16081692·>
• Wilkinson R, Lyons AB, Roberts D, Wong MX, Bartley PA, Jackson DE: Platelet endothelial cell adhesion molecule‑1 (PECAM‑1/CD31 acts as a regulator of B‑cell development, B‑cell antigen receptor (BCR)‑mediated activation, and autoimmune disease. Blood. 2002·Jul·1; 100(1):184‑9. <·PMID:·12759520·>
• Wong MX, Hayball JD, Jackson DE: PECAM‑1‑regulated signalling thresholds control tolerance in anergic transgenic B‑cells. Mol·Immunol. 2008·Mar; 45(6):1767‑81. <·PMID:·17977600·>
• Woodfin A, Voisin MB, Nourshargh S: PECAM‑1: a multi‑functional molecule in inflammation and vascular biolog. Arterioscler·Thromb·Vasc·Biol. 2007·Dec; 27(12):2514‑23. <·PMID:·17872453·>