CyFlow CD195 PE (RUO)
| 品番 | AH378490 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD195 PE,T21/8 | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 推奨使用量 | - | ||
| 容量 | 1 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG1 PE (BV655324) |
||
| 反応性|交差吸着 | Human | レーザー | Blue, Green, Yellow |
| 抗原 | CD195, CCR5 | 最大蛍光波長 | 576 nm |
| クローン | T21/8 | 最大励起波長 | 496 nm, 565 nm |
| ホスト | Mouse | 標識/Format | PE |
| アイソタイプ | IgG1 | 研究分野 | Immunophenotyping | Signaling |
| クローナリティ | monoclonal | アプリケーション | - |
Anti-Hu CD195 PE,T21/8
特異性
The mouse monoclonal antibody T21/8 recognizes the N-teminus of CD195 antigen, a 45 kDa G-protein coupled receptor 1 family protein expressed on resting T cells, monocytes, macrophages, and immature dendritic cells.
抗原情報
CD195 (CCR5, CKR-5) is a receptor for inflammatory CC-chemokines (characterized by a pair of adjacent cysteine residues), such as MIP-1 α, MIP-1 β, or RANTES. It is a G protein-associated seven-pass transmembrane protein expressed on resting T cells with memory/effector phenotype, monocytes, macrophages and immature dendritic cells. This chemokine receptor regulates the activation and directed migration of leukocytes. Importantly, along with CD4, CD195 functions as a major receptor for HIV. Their ligand is the viral glycoprotein gp120.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Hüttenrauch F, Pollok-Kopp B, Oppermann M: G protein‑coupled receptor kinases promote phosphorylation and beta‑arrestin‑mediated internalization of CCR5 homo‑ and hetero‑oligomers. J·Biol·Chem. 2005·Nov·11; 280(45):37503‑15. <·PMID:·16144840·>
• Mascalchi P, Lamort AS, Salomé L, Dumas F: Single Particle Tracking reveals two distinct environments for CD4 receptors at the surface of living T lymphocytes. Biochem·Biophys·Res·Commun. 2012·Jan·6; 417(1):409‑13. <·PMID:·22166195·>
• Monde K, Maeda Y, Tanaka Y, Harada S, Yusa K: Gp120 V3‑dependent impairment of R5 HIV‑1 infectivity due to virion‑incorporated CCR5. J·Biol·Chem. 2007·Dec·21; 282(51):36923‑32. <·PMID:·17971448·>
• Pollok-Kopp B, Schwarze K, Baradari VK, Oppermann M: Analysis of ligand‑stimulated CC chemokine receptor 5 (CCR5) phosphorylation in intact cells using phosphosite‑specific antibodies. J·Biol·Chem. 2003·Jan·24; 278(4):2190‑8. <·PMID:·12403770·>