CyFlow CD5 Pacific Blue (RUO)
| 品番 | AH799840 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD5 PAC BLUE,CRIS1 | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 推奨使用量 | - | ||
| 容量 | 0.4 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG2a Pacific Blue™ (CT002682) Mouse IgG2a Pacific Blue™ (AU451907) |
||
| 反応性|交差吸着 | Human | レーザー | Violet |
| 抗原 | CD5, Leu-1 | 最大蛍光波長 | 455 nm |
| クローン | CRIS1 | 最大励起波長 | 400 to 410 nm |
| ホスト | Mouse | 標識/Format | Pacific Blue™ |
| アイソタイプ | IgG2a | 研究分野 | Immunophenotyping |
| クローナリティ | monoclonal | アプリケーション | - |
Anti-Hu CD5 PAC BLUE,CRIS1
特異性
The mouse monoclonal antibody CRIS1 recognizes CD5 antigen, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes).
抗原情報
CD5 (T1) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains. The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies.Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (Rheumatoid Arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Alberola-Ila J, Places L, Cantrell DA, Vives J, Lozano F: Intracellular events involved in CD5‑induced human T cell activation and proliferation. J·Immunol. 1992·Mar·1; 148(5):1287‑93. <·PMID:·1371522·>
• Arrizabalaga P, Mirapeix E, Darnell A, Torras A, Revert L: Cellular immunity analysis using monoclonal antibodies in human glomerulonephritis. Nephron. 1989; 53(1):41‑9. <·PMID:·2789343·>
• Freedman AS, Freeman G, Whitman J, Segil J, Daley J, Levine H, Nadler LM: Expression and regulation of CD5 on in vitro activated human B cells. Eur·J·Immunol. 1989·May; 19(5):849‑55. <·PMID:·2472277·>
• Guarne A, Bravo J, Calvo J, Lozano F, Vives J, Fita I: Conformation of the hypervariable region L3 without the key proline residue. Protein·Sci. 1996·Jan; 5(1):167‑9. <·PMID:·8771210·>
• Raman C: CD5, an important regulator of lymphocyte selection and immune tolerance. Immunol·Res. 2002; 26(1‑3):255‑63. <·PMID:·12403363·>
• McMichael AJ, Beverley PCL, Cobbold S, et al (Eds): Leucocyte Typing III, White Cell Differentiation Antigens. Oxford·University·Press,·Oxford. 1987; 1‑1050. <·NLM·ID:·8913266·>