CyFlow CD64 Biotin (RUO)
| 品番 | AM698743 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD64 BIOTIN,10.1 | ||
| 包装単位 | 0.1 mg | ||
| 濃度 | 1 mg/ml | ||
| 推奨使用量 | - | ||
| 容量 | 0.1 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG1 Biotin (AQ807476) |
||
| 反応性|交差吸着 | Human | Non-Human Primates | レーザー | - |
| 抗原 | CD64, FcγRI | 最大蛍光波長 | - |
| クローン | 10.1 | 最大励起波長 | - |
| ホスト | Mouse | 標識/Format | Biotin |
| アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
| クローナリティ | monoclonal | アプリケーション | - |
Anti-Hu CD64 BIOTIN,10.1
特異性
The mouse monoclonal antibody 10.1 recognizes α subunit of CD64 antigen, a 72 kDa single chain type I glycoprotein, that is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes.
抗原情報
CD64 (FcγRI) is a cell surface receptor for Fc region of IgG. It is composed of specific ligand binding α subunit and promiscuous γ subunit, which is indispensable for tyrosine-based signaling. However, even the α subunit can transduce signals leading to cellular effector functions. The isoform FcγRIa1 binds human IgG with high affinity, has limited myeloid cell distribution, and a relatively large intracellular domain. Products of related genes include FcγRIb and FcγRIc isoforms, but these specify low affinity IgG receptors if functionally expressed at all. Besides a role in antigen clearance, FcgammaRI (a1) can potently enhance MHC class I and II antigen presentation in vitro and in vivo.
利用方法
The reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Working concentrations should be determined by the investigator.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Beekman JM, Bakema JE, van der Linden J, Tops B, Hinten M, van Vugt M, van de Winkel JG, Leusen JH: Modulation of FcgammaRI (CD64) ligand binding by blocking peptides of periplakin. J·Biol·Chem. 2004·Aug·6; 279(32):33875‑81. <·PMID:·15161926·>
• Brichard B, Varis I, Latinne D, Deneys V, de Bruyere M, Leveugle P, Cornu G: Intracellular cytokine profile of cord and adult blood monocytes. Bone·Marrow·Transplant. 2001·May; 27(10):1081‑6. <·PMID:·11438825·>
• Devaraj S, Davis B, Simon SI, Jialal I: CRP promotes monocyte‑endothelial cell adhesion via Fcgamma receptors in human aortic endothelial cells under static and shear flow conditions. Am·J·Physiol·Heart·Circ·Physiol. 2006·Sep; 291(3):H1170‑6. <·PMID:·16603696·>
• Devaraj S, Du Clos TW, Jialal I: Binding and internalization of C‑reactive protein by Fcgamma receptors on human aortic endothelial cells mediates biological effects. Arterioscler·Thromb·Vasc·Biol. 2005·Jul; 25(7):1359‑63. <·PMID:·15860734·>
• Dougherty GJ, Selvendran Y, Murdoch S, Palmer DG, Hogg N: The human mononuclear phagocyte high‑affinity Fc receptor, FcRI, defined by a monoclonal antibody, 10.1. Eur·J·Immunol. 1987·Oct; 17(10):1453‑9. <·PMID:·3500057·>
• Fadlon E, Vordermeier S, Pearson TC, Mire-Sluis AR, Dumonde DC, Phillips J, Fishlock K, Brown KA: Blood polymorphonuclear leukocytes from the majority of sickle cell patients in the crisis phase of the disease show enhanced adhesion to vascular endothelium and increased expression of CD64. Blood. 1998·Jan·1; 91(1):266‑74. <·PMID:·9414294·>
• Hashimoto S, Yamada M, Motoyoshi K, Akagawa KS: Enhancement of macrophage colony‑stimulating factor‑induced growth and differentiation of human monocytes by interleukin‑10. Blood. 1997·Jan·1; 89(1):315‑21. <·PMID:·8978307·>
• Jayaram Y, Buckle AM, Hogg N: The Fc receptor, FcRI, and other activation molecules on human mononuclear phagocytes after treatment with interferon‑gamma. Clin·Exp·Immunol. 1989·Mar; 75(3):414‑20. <·PMID:·2495203·>
• Roura-Mir C, Wang L, Cheng TY, Matsunaga I, Dascher CC, Peng SL, Fenton MJ, Kirschning C, Moody DB: Mycobacterium tuberculosis regulates CD1 antigen presentation pathways through TLR‑2. J·Immunol. 2005·Aug·1; 175(3):1758‑66. <·PMID:·16034117·>
• Sánchez-Torres C, García-Romo GS, Cornejo-Cortés MA, Rivas-Carvalho A, Sánchez-Schmitz G: CD16+ and CD16‑ human blood monocyte subsets differentiate in vitro to dendritic cells with different abilities to stimulate CD4+ T cells. Int·Immunol. 2001·Dec; 13(12):1571‑81. <·PMID:·11717198·>