CyFlow CD203c APC (RUO)
| 品番 | AQ234700 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD203C APC,NP4D6 | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 推奨使用量 | - | ||
| 容量 | 1 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG1 APC (AB294043) |
||
| 反応性|交差吸着 | Human | レーザー | Red |
| 抗原 | CD203c, E-NPP3 | 最大蛍光波長 | 660 nm |
| クローン | NP4D6 | 最大励起波長 | 650 nm |
| ホスト | Mouse | 標識/Format | APC |
| アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
| クローナリティ | monoclonal | アプリケーション | - |
Anti-Hu CD203C APC,NP4D6
特異性
The mouse monoclonal antibody NP4D6 recognizes CD203c antigen, a transmembrane ectoenzyme expressed on basophils and mast cells, and overexpressed upon their activation.
抗原情報
CD203c (ENPP-3) is integral membrane ectoenzyme (ectonucleotide pyrophosphatase/phosphodiesterase 3), that hydrolyses nucleotide triphosphates and thus modulates purinergic signaling. CD203c is expressed mainly on activated basophils and mast cells. CD203c is upregulated in response to IgE-receptor cross-linking and is overexpressed on neoplastic mast cells in patients with systemic mastocytosis. Measurement of its induced enhancement on the plasma membrane is useful for diagnostics of allergies.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Bühring HJ, Seiffert M, Giesert C, Marxer A, Kanz L, Valent P, Sano K: The basophil activation marker defined by antibody 97A6 is identical to the ectonucleotide pyrophosphatase/phosphodiesterase 31. Blood. 2001·May·15; 97(10):3303‑5. <·PMID:·11342463·>
• Bühring HJ, Simmons PJ, Pudney M, Müller R, Jarrossay D, van Agthoven A, Willheim M, Brugger W, Valent P, Kanz L: The monoclonal antibody 97A6 defines a novel surface antigen expressed on human basophils and their multipotent and unipotent progenitors. Blood. 1999·Oct·1; 94(7):2343‑56. <·PMID:·10498606·>
• Chirumbolo S, Brizzi M, Ortolani R, Vella A, Bellavite P: Inhibition of CD203c membrane up‑regulation in human basophils by high dilution of histamine: a controlled replication study. Inflamm·Res. 2009·Nov; 58(11):755‑64. <·PMID:·19418203·>
• Hauswirth AW, Escribano L, Prados A, Nuñez R, Mirkina I, Kneidinger M, Florian S, Sonneck K, Vales A, Schernthaner GH, Sanchez-Muñoz L, Sperr WR, Bühring HJ, Orfao A, Valent P: CD203c is overexpressed on neoplastic mast cells in systemic mastocytosis and is upregulated upon IgE receptor cross‑linking. Int·J·Immunopathol·Pharmacol. 2008·Oct-Dec; 21(4):797‑806. <·PMID:·19144265·>
• Platz IJ, Binder M, Marxer A, Lischka G, Valent P, Bühring HJ: Hymenoptera‑venom‑induced upregulation of the basophil activation marker ecto‑nucleotide pyrophosphatase/phosphodiesterase 3 in sensitized individuals. Int·Arch·Allergy·Immunol. 2001·Dec; 126(4):335‑42. <·PMID:·11815741·>
• Tokuda R, Nagao M, Hiraguchi Y, Hosoki K, Matsuda T, Kouno K, Morita E, Fujisawa T: Antigen‑induced expression of CD203c on basophils predicts IgE‑mediated wheat allergy. Allergol·Int. 2009·Jun; 58(2):193‑9. <·PMID:·19240377·>
• Wanich N, Nowak-Wegrzyn A, Sampson HA, Shreffler WG: Allergen‑specific basophil suppression associated with clinical tolerance i patients with milk allergy. J·Allergy·Clin·Immunol. 2009·Apr; 123(4):789‑94e20. <·PMID:·19348919·>