特異性

The mouse monoclonal antibody MEM-M6/2 recognizes extracellular Ig domain D1 of CD147 antigen, a 50-60 kDa type I transmembrane glycoprotein primarily expressed on all leukocytes, red blood cells, platelets and endothelial cells; it is not expressed by resting lymphocytes. The antibody MEM-M6/1 is a high-affinity antibody capable of binding to unstimulated peripheral blood T cells.

抗原情報

CD147 (basigin, neurothelin, OX-47, 5A11, CE9, M6) also known as EMMPRIN (extracellular matrix metalloproteinase inducer) or TCSF (tumour cell-derived collagenase-stimulatory factor) is an ubiquitously expressed cell surface protein with multiple glycosylated forms. The highest level of CD147 expression is on metabolically active cells, such as lymphoblasts, inflammatory cells, brown adipocytes and malignant tumor cells. CD147 has multiple functions, including facilitating of cell surface expression of monocarboxylate transporter proteins and extracellular matrix metalloproteinases, regulation of integrin functions, it plays roles in cell development and activation, fetal development or retinal function.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Iacono KT, Brown AL, Greene MI, Saouaf SJ: CD147 immunoglobulin superfamily receptor function and role in pathology. Exp·Mol·Pathol. 2007·Dec; 83(3):283‑95. <·PMID:·17945211·>
• Kirk P, Wilson MC, Heddle C, Brown MH, Barclay AN, Halestrap AP: CD147 is tightly associated with lactate transporters MCT1 and MCT4 and facilitates their cell surface expression. EMBO·J. 2000·Aug·1; 19(15):3896‑904. <·PMID:·10921872·>
• Koch C, Staffler G, Huttinger R, Hilgert I, Prager E, Cerny J, Steinlein P, Majdic O, Horejsi V, Stockinger H: T cell activation‑associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density. Int·Immunol. 1999·May; 11(5):777‑86. <·PMID:·10330283·>
• Melchior A, Denys A, Deligny A, Mazurier J, Allain F: Cyclophilin B induces integrin‑mediated cell adhesion by a mechanism involving CD98‑dependent activation of protein kinase C‑delta and p44/42 mitogen‑activated protein kinases. Exp·Cell·Res. 2008·Feb·1; 314(3):616‑28. <·PMID:·18054915·>
• Plánka L, Necas A, Srnec R, Rauser P, Starý D, Jancár J, Amler E, Filová E, Hlucilová J, Kren L, Gál P: Use of allogenic stem cells for the prevention of bone bridge formation in miniature pigs. Physiol·Res. 2009; 58(6):885‑93. <·PMID:·19093735·>
• Ruiz S, Castro-Castro A, Bustelo XR: CD147 Inhibits the Nuclear Factor of Activated T‑cells by Impairing Vav1 and Rac1 Downstream Signaling. J·Biol·Chem. 2008·Feb·29; 283(9):5554‑66. <·PMID:·18160397·>
• Schmidt R, Bültmann A, Fischel S, Gillitzer A, Cullen P, Walch A, Jost P, Ungerer M, Tolley ND, Lindemann S, Gawaz M, Schömig A, May AE: Extracellular matrix metalloproteinase inducer (CD147 is a novel receptor on platelets, activates platelets, and augments nuclear factor kappaB‑dependent inflammation in monocytes. Circ·Res. 2008·Feb·15; 102(3):302‑9. <·PMID:·18048771·>
• Wilson MC, Meredith D, Fox JE, Manoharan C, Davies AJ, Halestrap AP: Basigin (CD147 is the target for organomercurial inhibition of monocarboxylate transporter isoforms 1 and 4: the ancillary protein for the insensitive MCT2 is EMBIGIN (gp70). J·Biol·Chem. 2005·Jul·22; 280(29):27213‑21. <·PMID:·15917240·>
• Xu D, Hemler ME: Metabolic activation‑related CD147‑CD98 complex. Mol·Cell·Proteomics. 2005·Aug; 4(8):1061‑71. <·PMID:·15901826·>