特異性

The mouse monoclonal antibody MEM-04 recognizes CD50 antigen, a 120-130 kDa type I membrane protein (immunoglobulin supergene family) expressed on leukocytes, endothelial cells and Langerhans cells; it is negative on platelets and erythrocytes. The MEM-04 blocks DC-SIGN (CD209) binding to ICAM-3 (epitope in D1).

抗原情報

CD50 ( ICAM-3; intracellular adhesion molecule 3) is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA)-1 integrin. Besides functioning as an adhesive molecule that mediates e.g. the contact between T cells and antigen presenting cells, ICAM-3 regulates affinity of LFA-1 for ICAM-1 and induces T cell activation and proliferation. ICAM-3 plays an essential role in the initiation of the immune response both on T cells and antigen presenting cells and interacts also with CD209 (DC-SIGN; dendritic cell-specific ICAM-3-grabbing nonintegrin), a C-type lectin of dendritic cells and macrophages; this process is involved in dialogue between dendritic cells and granulocytes.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Arroyo AG, Campanero MR, Sánchez-Mateos P, Zapata JM, Ursa MA, del Pozo MA, Sánchez-Madrid F: Induction of tyrosine phosphorylation during ICAM‑3 and LFA‑1‑mediated intercellular adhesion, and its regulation by the CD45 tyrosine phosphatase. J·Cell·Biol. 1994·Sep; 126(5):1277‑86. <·PMID:·7520448·>
• Bogoevska V, Nollau P, Lucka L, Grunow D, Klampe B, Uotila LM, Samsen A, Gahmberg CG, Wagener C: DC‑SIGN binds ICAM‑3 isolated from peripheral human leukocytes through Lewis x residues. Glycobiology. 2007·Mar; 17(3):324‑33. <·PMID:·17145745·>
• Campanero MR, del Pozo MA, Arroyo AG, Sánchez-Mateos P, Hernández-Caselles T, Craig A, Pulido R, Sánchez-Madrid F: ICAM‑3 interacts with LFA‑1 and regulates the LFA‑1/ICAM‑1 cell adhesion pathway. J·Cell·Biol. 1993·Nov; 123(4):1007‑16. <·PMID:·7901223·>
• Hernandez-Caselles T, Rubio G, Campanero MR, del Pozo MA, Muro M, Sanchez-Madrid F, Aparicio P: ICAM‑3, the third LFA‑1 counterreceptor, is a co‑stimulatory molecule for both resting and activated T lymphocytes. Eur·J·Immunol. 1993·Nov; 23(11):2799‑806. <·PMID:·8223855·>