特異性

The mouse monoclonal antibody MEM-E/07 recognizes native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia): HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly).

抗原情報

利用方法

The reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 2 µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Lo Monaco E, Tremante E, Cerboni C, Melucci E, Sibilio L, Zingoni A, Nicotra MR, Natali PG, Giacomini P: Human leukocyte antigen E contributes to protect tumor cells from lysis by natural killer cells. Neoplasia. 2011·Sep; 13(9):822‑30. <·PMID:·21969815·>
• Nachmani D, Zimmermann A, Oiknine Djian E, Weisblum Y, Livneh Y, Khanh Le VT, Galun E, Horejsi V, Isakov O, Shomron N, Wolf DG, Hengel H, Mandelboim O: MicroRNA editing facilitates immune elimination of HCMV infected cells. PLoS·Pathog. 2014·Feb·27; 10(2):e1003963. <·PMID:·24586166·>
• Palmisano GL, Contardi E, Morabito A, Gargaglione V, Ferrara GB, Pistillo MP: HLA‑E surface expression is independent of the availability of HLA class I signal sequence‑derived peptides in human tumor cell lines. Hum·Immunol. 2005·Jan; 66(1):42705. <·PMID:·15620456·>
• Sibilio L, et al.: Abstract 45 from the 3rd International Conference on HLA‑G; Paris, July 2003. Tissue·Antigens. 2003; 62:339. <·No·PMID·>