CyFlow CD150 FITC (RUO)
| 品番 | AW692255 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD150 FITC,SLAM.4 | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 推奨使用量 | - | ||
| 容量 | 0.4 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG1 FITC (CD051682) |
||
| 反応性|交差吸着 | Human | レーザー | Blue |
| 抗原 | CD150, SLAMF1, IPO-3 | 最大蛍光波長 | 518 nm |
| クローン | SLAM.4 | 最大励起波長 | 490 to 495 nm |
| ホスト | Mouse | 標識/Format | FITC |
| アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
| クローナリティ | monoclonal | アプリケーション | - |
Anti-Hu CD150 FITC,SLAM.4
特異性
The mouse monoclonal antibody SLAM.4 recognizes CD150 antigen, a cell surface molecule expressed on lymphocytes and involved in their activation.
抗原情報
CD150 (SLAM; signaling lymphocyte activation molecule) is a 70-95 kDa single chain transmembrane phosphoglycoprotein of the CD2 family. Its extracellular part contains eight potential N-glycosylation sites, and the intracellular tail contains three unique tyrosine-based motifs. These binding sites can be recognized by SH2-binding phosphatases and the adaptor proteins, such as SAP/SH2D1A or EAT-2. The SLAM family receptors are involved in leukocytes activation and contribute to the effective germinal center formation, generation of high-affinity antibody-secreting plasma cells, and memory T and B cells, thereby facilitating long-term immune response. CD150 expression is upregulated after cell activation.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• De Salort J, Sintes J, Llinás L, Matesanz-Isabel J, Engel P: Expression of SLAM (CD150) cell‑surface receptors on human B‑cell subsets: from pro‑B to plasma cells. Immunol·Lett. 2011·Jan·30; 134(2):129‑36. <·PMID:·20933013·>
• García VE, Quiroga MF, Ochoa MT, Ochoa L, Pasquinelli V, Fainboim L, Olivares LM, Valdez R, Sordelli DO, Aversa G, Modlin RL, Sieling PA: Signaling lymphocytic activation molecule expression and regulation in human intracellular infection correlate with Th1 cytokine patterns. J·Immunol. 2001·Nov·15; 167(10):5719‑24. <·PMID:·11698444·>
• Romero X, Benítez D, March S, Vilella R, Miralpeix M, Engel P: Differential expression of SAP and EAT‑2‑binding leukocyte cell‑surface molecules CD84, CD150 (SLAM), CD229 (Ly9) and CD244 (2B4). Tissue·Antigens. 2004·Aug; 64(2):132‑44. <·PMID:·15245368·>
• Romero X, Zapater N, Calvo M, Kalko SG, de la Fuente MA, Tovar V, Ockeloen C, Pizcueta P, Engel P: CD229 (Ly{09} lymphocyte cell surface receptor interacts homophilically through its N‑terminal domain and relocalizes to the immunological synapse. J·Immunol. 2005·Jun·1; 174(11):7033‑42. <·PMID:·15905546·>