特異性

The mouse monoclonal antibody NAP-07 recognizes Non-T cell activation linker (NTAL), also known as linker of activated B cells (LAB), a 25 - 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B lymphocytes, NK cells and myeloid cells.

抗原情報

NTAL (Non-T cell activation linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Brdicka T, Imrich M, Angelisova P, Brdickova N, Horvath O, Spicka J, Hilgert I, Luskova P, Draber P, Novak P, Engels N, Wienands J, Simeoni L, Osterreicher J, Aguado E, Malissen M, Schraven B, Horejsi V: Non‑T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling. J·Exp·Med. 2002·Dec·16; 196(12):1617‑26. <·PMID:·12486104·>
• Lebduska P, Korb J, Tůmová M, Heneberg P, Dráber P: Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non‑adherent mast cells. J·Immunol·Methods. 2007·Dec·1; 328(1‑2):139‑51. <·PMID:·17900607·>
• Tkaczyk C, Horejsi V, Iwaki S, Draber P, Samelson LE, Satterthwaite AB, Nahm DH, Metcalfe DD, Gilfillan AM: NTAL phosphorylation is a pivotal link between the signaling cascades leading to human mast cell degranulation following Kit activation and Fc epsilon RI aggregation. Blood. 2004·Jul·1; 104(1):207‑14. <·PMID:·15010370·>
• Volna P, Lebduska P, Draberova L, Simova S, Heneberg P, Boubelik M, Bugajev V, Malissen B, Wilson BS, Horejsi V, Malissen M, Draber P: Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL. J·Exp·Med. 2004·Oct·18; 200(8):1001‑13. <·PMID:·15477348·>
• Yamasaki S, Ishikawa E, Sakuma M, Kanagawa O, Cheng AM, Malissen B, Saito T: LAT and NTAL mediate immunoglobulin E‑induced sustained extracellularsignal‑regulated kinase activation critical for mast cell survival. Mol·Cell·Biol. 2007·Jun; 27(12):4406‑15. <·PMID:·17420272·>