特異性

The mouse monoclonal antibody dG9 (δG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells.

抗原情報

Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Hersperger AR1, Makedonas G, Betts MR: Flow cytometric detection of perforin upregulation in human CD8 T cells. Cytometry·A. 2008·Nov; 73(11):1050‑7. <·PMID:·18615597·>
• Qiu Y, Chen J, Liao H, Zhang Y, Wang H, Li S, Luo Y, Fang D, Li G, Zhou B, Shen L, Chen CY, Huang D, Cai J, Cao K, Jiang L, Zeng G, Chen ZW: Tim‑3‑expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti‑TB effector functions. PLoS·Pathog. 2012; 8(11):e1002984. <·PMID:·23144609·>
• Takeuchi M, Inoue T, Otani T, Yamasaki F, Nakamura S, Kibata M: Cell‑in‑cell structures formed between human cancer cell lines and the cytotoxic regulatory T‑cell line HOZOT. J·Mol·Cell·Biol. 2010·Jun; 2(3):139‑51. <·PMID:·20348186·>
• Vargas-Inchaustegui DA, Demberg T, Robert-Guroff M: A CD8α(‑) subpopulation of macaque circulatory natural killer cells can mediate both antibody‑dependent and antibody‑independent cytotoxic activities. Immunology. 2011·Nov; 134(3):326‑40. <·PMID:·21978002·>