CyFlow HLA-Class I PE (RUO)
| 品番 | BE648498 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu HLA-ClAss I PE,MEM-147 | ||
| 包装単位 | 0.1 mg | ||
| 濃度 | 0.1 mg/ml | ||
| 推奨使用量 | - | ||
| 容量 | 1 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG1 PE (BV655324) |
||
| 反応性|交差吸着 | Human | レーザー | Blue, Green, Yellow |
| 抗原 | HLA-Class I | 最大蛍光波長 | 576 nm |
| クローン | MEM-147 | 最大励起波長 | 496 nm, 565 nm |
| ホスト | Mouse | 標識/Format | PE |
| アイソタイプ | IgG1 | 研究分野 | MHC |
| クローナリティ | monoclonal | アプリケーション | - |
Anti-Hu HLA-ClAss I PE,MEM-147
特異性
The mouse monoclonal antibody MEM-147 recognizes all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types. The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.
抗原情報
HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant β2-microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumor defense. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
利用方法
The reagent is designed for Flow Cytometry analysis. Suggested working usage is 5 µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single‑molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int·Immunol. 2007·May; 19(5):675‑84. <·PMID:·17446208·>
• Ilangumaran S, Briol A, Hoessli DC: CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid‑rich plasma membrane domains of human peripheral blood lymphocytes. Blood. 1998·May·15; 91(10):3901‑8. <·PMID:·9573028·>
• Tran TM, Ivanyi P, Hilgert I, Brdicka T, Pla M, Breur B, Flieger M, Ivaskova E, Horejsi V: The epitope recognized by pan‑HLA class I‑reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA‑B27/beta2‑microglobulin complex. Immunogenetics. 2001·Aug; 53(6):440‑6. <·PMID:·11685454·>