シスメックス株式会社

研究用フローサイトと理化学分析

研究用フローサイトと理化学分析

CyFlow CK-19 Alexa Fluor 488 (RUO)

品番 BF824641
抗体名 Anti-Hu CK-19 AF 488,A53-B/A2
包装単位 0.1 mg
濃度 0.1 mg/ml
推奨使用量 -
容量 1 ml
関連製品
(アイソタイプコントロール)
Mouse IgG2a Alexa Fluor™ 488 (BF508817)
Mouse IgG2a Alexa Fluor™ 488 (BN791562)
反応性|交差吸着 Human レーザー Blue
抗原 Cytokeratin 19 最大蛍光波長 519 nm
クローン A53-B/A2 最大励起波長 495 nm
ホスト Mouse 標識/Format Alexa Fluor™ 488
アイソタイプ IgG2a 研究分野 Cytoskeleton
クローナリティ monoclonal アプリケーション

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Anti-Hu CK-19 AF 488,A53-B/A2

特異性

The mouse monoclonal antibody A53-B/A2 recognizes Rod domain of cytokeratin 19 (40 kDa) in human tissue. Cytokeratin 19 is not expressed in hepatocytes; it is often co-expressed with cytokeratin 7.

抗原情報

Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

利用方法

The reagent is designed for Flow Cytometry analysis. Suggested working usage is 10 µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Bártek J, Bártková J, Taylor-Papadimitriou J, Rejthar A, Kovarík J, Lukás Z, Vojtĕsek B: Differential expression of keratin 19 in normal human epithelial tissues revealed by monospecific monoclonal antibodies. Histochem·J. 1986·Oct; 18(10):565‑75. <·PMID:·2433255·>
• Karsten U, Papsdorf G, Roloff G, Stolley P, Abel H, Walther I, Weiss H: Monoclonal anti‑cytokeratin antibody from a hybridoma clone generated by electrofusion. Eur·J·Cancer·Clin·Oncol. 1985·Jun; 21(6):733‑40. <·PMID:·2410280·>
• Kasper M, Moll R, Stosiek P, Karsten U: Patterns of cytokeratin and vimentin expression in the human eye. Histochemistry. 1988; 89(4):369‑77. <·PMID:·2457569·>