CyFlow CD5 Biotin (RUO)
| 品番 | BH589141 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD5 BIOTIN,L17F12 | ||
| 包装単位 | 0.1 mg | ||
| 濃度 | 1 mg/ml | ||
| 推奨使用量 | - | ||
| 容量 | 0.1 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG2a Biotin (BH298720) |
||
| 反応性|交差吸着 | Human | レーザー | - |
| 抗原 | CD5, Leu-1 | 最大蛍光波長 | - |
| クローン | L17F12 | 最大励起波長 | - |
| ホスト | Mouse | 標識/Format | Biotin |
| アイソタイプ | IgG2a | 研究分野 | Immunophenotyping |
| クローナリティ | monoclonal | アプリケーション | - |
Anti-Hu CD5 BIOTIN,L17F12
特異性
The mouse monoclonal antibody L17F12 recognizes CD5 antigen, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes).
抗原情報
CD5 (T1) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains. The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies.Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (Rheumatoid Arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.
利用方法
The reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Working concentrations should be determined by the investigator.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Dunphy CH, Tang W: The value of CD64 expression in distinguishing acute myeloid leukemia with monocytic differentiation from other subtypes of acute myeloid leukemia: a flow cytometric analysis of 64 cases. Arch·Pathol·Lab·Med. 2007·May; 131(5):748‑54. <·PMID:·17488160·>
• Engleman EG, Warnke R, Fox RI, Dilley J, Benike CJ, Levy R: Studies of a human T lymphocyte antigen recognized by a monoclonal antibody. Proc·Natl·Acad·Sci·USA. 1981·Mar; 78(3):1791‑5. <·PMID:·7015346·>
• Gong JZ, Lagoo AS, Peters D, Horvatinovich J, Benz P, Buckley PJ: Value of CD23 determination by flow cytometry in differentiating mantle cell lymphoma from chronic lymphocytic leukemia/small lymphocytic lymphoma. Am·J·Clin·Pathol. 2001·Dec; 116(6):893‑7. <·PMID:·11764079·>
• McAlister MS, Davis B, Pfuhl M, Driscoll PC: NMR analysis of the N‑terminal SRCR domain of human CD5: engineering of a glycoprotein for superior characteristics in NMR experiments. Protein·Eng. 1998·Oct; 11(10):847‑53. <·PMID:·9862202·>
• Shuster JJ, Falletta JM, Pullen DJ, Crist WM, Humphrey GB, Dowell BL, Wharam MD, Borowitz M: Prognostic factors in childhood T‑cell acute lymphoblastic leukemia: a Pediatric Oncology Group study. Blood. 1990·Jan·1; 75(1):166‑73. <·PMID:·1688495·>