特異性

The mouse monoclonal antibody 4H10 recognizes human immunoglobulin E (IgE); it recognizes an epitope different from the ones recognized by BE5 and 4G7 antibodies to IgE.

抗原情報

Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Balogh Z, Merétey K, Falus A, Bozsóky S: Serological abnormalities in juvenile chronic arthritis: a review of 46 cases. Ann·Rheum·Dis. 1980·Apr; 39(2):129‑34. <·PMID:·6966908·>
• Brinkmann V, Heusser CH: T cell‑dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell·Immunol. 1993·Dec; 152(2):323‑32. <·PMID:·8258141·>
• Franklin EC: Structure and function of immunoglobulins. Acta·Endocrinol·Suppl·(Copenh). 1975; 194:77‑95. <·PMID:·47690·>
• Fuller JM, Keyser JW: Serum immunoglobulins after surgical operation. Clin·Chem. 1975·May; 21(6):667‑71. <·PMID:·1122610·>
• Gould HJ, Beavil RL, Vercelli D: IgE isotype determination: epsilon‑germline gene transcription, DNA recombination and B‑cell differentiation. Br·Med·Bull. 2000; 56(4):908‑24. <·PMID:·11359628·>