CyFlow CD137 PE (RUO)
| 品番 | BN858229 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD137 PE,4B4-1 | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 推奨使用量 | - | ||
| 容量 | 1 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG1 PE (BV655324) |
||
| 反応性|交差吸着 | Human | Non-Human Primates | レーザー | Blue, Green, Yellow |
| 抗原 | CD137, 4-1BB, TNFRSF9 | 最大蛍光波長 | 576 nm |
| クローン | 4B4-1 | 最大励起波長 | 496 nm, 565 nm |
| ホスト | Mouse | 標識/Format | PE |
| アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
| クローナリティ | monoclonal | アプリケーション | - |
Anti-Hu CD137 PE,4B4-1
特異性
The mouse monoclonal antibody 4B4-1 recognizes CD137 antigen, a 40 kDa type I transmembrane protein of the TNFR family expressed mainly on activated T cells.
抗原情報
CD137 (TNFRSF9, 4-1BB), is an inducible costimulatory molecule expressed mainly on activated T cells. Its ligand, known as 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells. CD137 signaling leads to maintaining the survival of activated T cells and CD8+ memory T cells, and clonal expansion of T cells, but also to suppressing myelopoiesis and dendritic cell development. Triggered CD137 induces a cytokine release profile regulating peripheral monocyte survival. Soluble forms of CD137 may provide negative control mechanism for some immune responses.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Bellarosa D, Bressan A, Bigioni M, Parlani M, Maggi CA, Binaschi M: SAHA/Vorinostat induces the expression of the CD137 receptor/ligand system and enhances apoptosis mediated by soluble CD137 receptor in a human breast cancer cell line. Int·J·Oncol. 2012·Oct; 41(4):1486‑94. <·PMID:·22797667·>
• Chan SL, Voskens CJ, Lin W, Schindler DG, Azimzadeh A, Wang LX, Taylor RJ, Strome SE, Schulze DH: Epitope mapping of a chimeric CD137 mAb: a necessary step for assessing the biologic relevance of non‑human primate models. J·Mol·Recognit. 2009·May-Jun; 22(3):242‑9. <·PMID:·19177494·>
• Fernández Do Porto DA, Jurado JO, Pasquinelli V, Alvarez IB, Aspera RH, Musella RM, García VE: CD137 differentially regulates innate and adaptive immunity against Mycobacterium tuberculosis. Immunol·Cell·Biol. 2012·Apr; 90(4):449‑56. <·PMID:·21747409·>
• Yi L, Zhao Y, Wang X, Dai M, Hellström KE, Hellström I, Zhang H: Human and mouse CD137 have predominantly different binding CRDs to their respective ligands. PLoS·One. 2014·Jan·21; 9(1):e86337. <·PMID:·24466035·>