特異性

The mouse monoclonal antibody EM-22 recognizes C-terminal part of β-catenin, a 88 kDa multifunctional protein involved both in cell adhesion and in activation of transcription.

抗原情報

β-catenin is a multifunctional protein involved both in cell adhesion and in activation of transcription. Calcium-dependent intercellular adhesion transmembrane glycoprotein E-cadherin interacts by its cytoplasmic domain with reciprocally bound α, β and γ catenin. β-catenin links this complex through α-actinin to the cytoskeleton. Functional cadherin-catenin system is important for invasiveness of tumor cells. β-catenin level in cytoplasm is controlled by glycogen synthase kinase-3 β. When activity of this kinase is blocked (e.g. by excessive stimulation of Wnt signaling pathway), hypophosphorylated stable form of β-catenin accumulates in the cytoplasm, translocates to the nucleus and activates transcription of genes including those that are involved in cell cycle control. As a result, cell division and neoplastic transformation are promoted.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Jung IM, Chung JK, Kim YA, Kim JE, Heo SC, Ahn YJ, Hwang KT, Kim BG, Lee KL, Kim CW, Kim WH, Chang MS: Epstein‑Barr Virus, Beta‑Catenin, and E‑cadherin in Gastric Carcinomas. J·Korean·Med·Sci. 2007·Oct; 22(5):855‑61. <·PMID:·17982235·>
• Nowak M, Madej JA, Dziegiel P: Expression of E‑cadherin, beta‑catenin and Ki‑67 antigen and their reciprocal relationships in mammary adenocarcinomas in bitches. Folia·Histochem·Cytobiol. 2007; 45(3):233‑8. <·PMID:·17951173·>
• Valenta T, Lukas J, Korinek V: HMG box transcription factor TCF‑4's interaction with CtBP1 controls the expression of the Wnt target Axin2/Conductin in human embryonic kidney cells. Nucleic·Acids·Res. 2003·May·1; 31(9):2369‑80. <·PMID:·12711682·>