特異性

The mouse monoclonal antibody MEM-E/06 recognizes native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. The published results showed that the antibody cross-reacts with some classical MHC Class I molecules (HLA-A3, -A11, -B7). However, the Workshop I Session on the 3rd International Conference on HLA-G (Paris, July 2003) confirmed that the antibody exhibits much broader cross-reactivity classical MHC Class I antigens, namely with HLA-A24, -A32, -B8, -B15, -B27, -B35, -B44, -B54, -C3, -C4, -C5, -C7.

抗原情報

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Dambaeva SV, Bondarenko GI, Grendell RL, Kravitz RH, Durning M, Golos TG: Non‑classical MHC‑E (Mamu‑E) expression in the rhesus monkey placenta. Placenta. 2008·Jan; 29(1):58‑70. <·PMID:·17996936·>
• Gonen-Gross T, Achdout H, Arnon TI, Gazit R, Stern N, Horejsí V, Goldman-Wohl D, Yagel S, Mandelboim O: The CD85J/leukocyte inhibitory receptor‑1 distinguishes between conformed and beta 2‑microglobulin‑free HLA‑G molecules. J·Immunol. 2005·Oct·15; 175(8):4866‑74. <·PMID:·16210588·>
• Lo Monaco E, Tremante E, Cerboni C, Melucci E, Sibilio L, Zingoni A, Nicotra MR, Natali PG, Giacomini P: Human leukocyte antigen E contributes to protect tumor cells from lysis by natural killer cells. Neoplasia. 2011·Sep; 13(9):822‑30. <·PMID:·21969815·>
• Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N: Characterization of monoclonal antibodies recognizing HLA‑G or HLA‑E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum·Immunol. 2003·Mar; 64(3):315‑26. <·PMID:·12590976·>
• Palmisano GL, Contardi E, Morabito A, Gargaglione V, Ferrara GB, Pistillo MP: HLA‑E surface expression is independent of the availability of HLA class I signal sequence‑derived peptides in human tumor cell lines. Hum·Immunol. 2005·Jan; 66(1):42705. <·PMID:·15620456·>