特異性

The mouse monoclonal antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human β3-tubulin.

抗原情報

The β3-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumors originating from these cells. The β3-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Draberova E, Lukas Z, Ivanyi D, Viklicky V, Draber P: Expression of class III beta‑tubulin in normal and neoplastic human tissues.. Histochem·Cell·Biol. 1998·Mar; 109(3):231‑9. <·PMID:·9541471·>
• Jirásek T, Cipro S, Musilová A, Kubecová M, Mandys V: Expression of class III beta‑tubulin in colorectal carcinomas: an immunohistochemical study using TU‑20&TuJ‑1 antibody. Indian·J·Med·Res. 2009·Jan; 129(1):89‑94. <·PMID:·19287064·>
• Jirasek T, Pisarikova E, Viklicky V, Mandys V: Expression of class III beta‑tubulin in malignant epithelial tumours: an immunohistochemical study using TU‑20 and TuJ‑1 antibodies. Folia·Histochem·Cytobiol. 2007; 45(1):41‑5. <·PMID:·17378245·>
• Katsetos CD, Dráberová E, Smejkalová B, Reddy G, Bertrand L, de Chadarévian J-P, Legido A, Nissanov J, Baas PW, Dráber P: Class III beta‑tubulin and gamma‑tubulin are co‑expressed and form complexes in human glioblastoma cells.. Neurochem·Res. 2007; 32:1387‑1398. <·PMID:·20162618·>
• Kukharskyy V, Sulimenko V, Macůrek L, Sulimenko T, Dráberová E, Dráber P: Complexes of gamma‑tubulin with nonreceptor protein tyrosine kinases Src and Fynin differentiating P19 embryonal carcinoma cells. Exp·Cell·Res. 2004·Aug·1; 298(1):218‑28. <·PMID:·15242776·>
• Peknicova J, Kubatova A, Sulimenko V, Draberova E, Viklicky V, Hozak P, Draber P: Differential subcellular distribution of tubulin epitopes in boar spermatozoa:recognition of class III beta‑tubulin epitope in sperm tail. Biol·Reprod. 2001·Sep; 65(3):672‑9. <·PMID:·11514327·>
• Theodorou E, Dalembert G, Heffelfinger C, White E, Weissman S, Corcoran L, Snyder M: A high throughput embryonic stem cell screen identifies Oct‑2 as a bifunctional regulator of neuronal differentiation. Genes·Dev. 2009·Mar·1; 23(5):575‑88. <·PMID:·19270158·>