CyFlow Kappa-LC Pacific Blue (RUO)
| 品番 | BW314376 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu KAPPA-LC PAC BLUE,TB28-2 | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 推奨使用量 | - | ||
| 容量 | 0.4 ml | ||
| 関連製品 (アイソタイプコントロール) |
- | ||
| 反応性|交差吸着 | Human | レーザー | Violet |
| 抗原 | κ light chains | 最大蛍光波長 | 455 nm |
| クローン | TB28-2 | 最大励起波長 | 400 to 410 nm |
| ホスト | Mouse | 標識/Format | Pacific Blue™ |
| アイソタイプ | IgG1 | 研究分野 | Immunoglobulins |
| クローナリティ | monoclonal | アプリケーション | - |
Anti-Hu KAPPA-LC PAC BLUE,TB28-2
特異性
The mouse monoclonal antibody TB28-2 recognizes human Ig κ light chains of both secreted and cell surface immunoglobulin. It detects also free κ light chains.
抗原情報
Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (κ and λ; both having a molecular weight of 22.5kDa). κ and λ consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of κ to λ is 70:30.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Böttcher S, Ritgen M, Buske S, Gesk S, Klapper W, Hoster E, Hiddemann W, Unterhalt M, Dreyling M, Siebert R, Kneba M, Pott C: EU MCL MRD Group: Minimal residual disease detection in mantle cell lymphoma: methods and significance of four‑color flow cytometry compared to consensus IGH‑polymerase chain reaction at initial staging and for follow‑up examinations. Haematologica. 2008·Apr; 93(4):551‑9. <·PMID:·18379010·>
• Jourdan M, Caraux A, De Vos J, Fiol G, Larroque M, Cognot C, Bret C, Duperray C, Hose D, Klein B: An in vitro model of differentiation of memory B cells into plasmablasts andplasma cells including detailed phenotypic and molecular characterization. Blood. 2009·Dec·10; 114(25):5173‑81. <·PMID:·19846886·>
• Karandikar NJ, Aquino DB, McKenna RW, Kroft SH: Transient myeloproliferative disorder and acute myeloid leukemia in Down syndrome: An immunophenotypic analysis. Am·J·Clin·Pathol. 2001·Aug; 116(2):204‑10. <·PMID:·11488066·>
• Kiyotaki M, Cooper MD, Bertoli LF, Kearney JF, Kubagawa H: Monoclonal anti‑Id antibodies react with varying proportions of human B lineage cells. J·Immunol. 1987·Jun·15; 138(12):4150‑8. <·PMID:·3495581·>
• Nakamura T, Kubagawa H, Cooper MD: Heterogeneity of immunoglobulin‑associated molecules on human B cells identified by monoclonal antibodies. Proc·Natl·Acad·Sci·USA. 1992·Sep·15; 89(18):8522‑6. <·PMID:·1382292·>