特異性

The mouse monoclonal antibody UP-R1 recognizes CD158f antigen, a 60 kDa glycoprotein serving as a HLA class I ligand, and mainly expressed on a subset of NK cells and a small population of T cells. Its expression is highly polymorphic between individuals.

抗原情報

CD158f (KIR2DL5) is a polymorphic 60 kDa transmembrane glycoprotein with two Ig-like extracellular domains by which it recognize HLA class I molecules. Its long intracellular domain contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that upon extracellular ligand-mediated phosphorylation serve as docking sites for inhibitory phosphatases, which results in blocking natural cytotoxicity as well as antibody-dependent cytotoxicity of the particular NK cell, and its adhesion toward target cells. Together with other killer inhibitory receptors CD158f is important for immunological tolerance to discriminate between normal and abnormal cells. Besides NK cells it is expressed on a small population of cytotoxic T cells. Expression of CD158f alleles is highly variable in the population.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Du Z, Sharma SK, Spellman S, Reed EF, Rajalingam R: KIR2DL5 alleles mark certain combination of activating KIR genes. Genes·Immun. 2008·Jul; 9(5):470‑80. <·PMID:·18509341·>
• Estefanía E, Flores R, Gómez-Lozano N, Aguilar H, López-Botet M, Vilches C: Human KIR2DL5 is an inhibitory receptor expressed on the surface of NK and T lymphocyte subsets. J·Immunol. 2007·Apr·1; 178(7):4402‑10. <·PMID:·17371997·>
• Yusa S, Catina TL, Campbell KS: KIR2DL5 can inhibit human NK cell activation via recruitment of Src homology region 2‑containing protein tyrosine phosphatase‑2 (SHP‑2). J·Immunol. 2004·Jun·15; 172(12):7385‑92. <·PMID:·15187115·>