CyFlow CD157 APC (RUO)
| 品番 | CB736754 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD157 APC,SY11B5 | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 推奨使用量 | - | ||
| 容量 | 1 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG1 APC (AB294043) |
||
| 反応性|交差吸着 | Human | Non-Human Primates | レーザー | Red |
| 抗原 | CD157, BST-1 | 最大蛍光波長 | 660 nm |
| クローン | SY11B5 | 最大励起波長 | 650 nm |
| ホスト | Mouse | 標識/Format | APC |
| アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
| クローナリティ | monoclonal | アプリケーション | - |
Anti-Hu CD157 APC,SY11B5
特異性
The mouse monoclonal antibody SY11B5 recognizes CD157 antigen, a 45 kDa GPI-anchored protein expressed mainly on monocytes, macrophages, granulocytes and bone marrow stromal cells.
抗原情報
CD157 (cADPr hydrolase 2) is a GPI-anchored ectoenzyme possessing ADP-ribosyl cyclase and cyclic ADP-ribose hydrolase activity. It uses NAD and cADP-ribose as substrates. CD157 is expressed on granulocytes, monocytes, macrophages, follicular dendritic cells, bone marrow stromal cells and human umbilical cord vein endothelial cells. In case of rheumatoid arthritis is expression is often higher and it is also differentially expressed in the myeloid leukemias. It may also have a signaling role.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Arriga R, Caratelli S, Coppola A, Spagnoli GC, Venditti A, Amadori S, Lanzilli G, Lauro D, Palomba P, Sconocchia T, Del Principe MI: Enhancement of anti-leukemia activity of NK cells in vitro and in vivo by inhibition of leukemia cell-induced NK cell damage. Oncotarget. 2016·Jan·12; 7(2):2070. <·PMID:·26655503·>
• Sutherland DR, Acton E, Keeney M, Davis BH, Illingworth A: Use of CD in FLAER‐based assays for high‐sensitivity PNH granulocyte and PNH monocyte detection. Cytometry·Part·B:·Clinical·Cytometry. 2014·Jan·1; 86(1):44-55. <·PMID:·23922226·>
• Sutherland R, Acton E, Keeney M, Borowitz M, Richards S, Fletcher M, Whitby L, Barnett D, Illingworth A: Recommended antibody clones and conjugates suitable for high-sensitivity flow cytometric detection of PNH Red (RBC) and White (WBC) Blood Cells. Cytometry·Part·B:·Clinical·Cytometry. 2013 Nov 1; 84(6):422-3. <·No·PMID·>
• Marinov I, Kohoutová M, Tkáčová V, Pešek A, Čermák J, Cetkovský P: Clinical relevance of CD for rapid and cost‐effective simultaneous evaluation of PNH granulocytes and monocytes by flow cytometry. Int·J·Lab·Hematol. 2015·Apr; 37(2):231-7. <·PMID:·24963875·>
• Marinov I, Illingworth AJ, Benko M, Sutherland DR: Performance characteristics of a non‐FLAER‐based PNH assay for simultaneous evaluation of PNH neutrophils and PNH monocytes by flow cytometry, following published PNH guidelines. Cytometry·B·Clin·Cytom. 2016·Jun·13; <·PMID:·27294344·>