特異性

The mouse monoclonal antibody CLB-GB11 recognizes granzyme B, a 31 kDa serine protease expressed in activated Tc cells and NK cells.

抗原情報

Granzyme B is a serine protease that is expressed in cytoplasmic granules of cytotoxic T lymphocytes and NK cells. Vectorial secretion of perforin and granzymes is responsible for their granule-mediated cytotoxicity. Granzyme B plays a pivotal role in the induction of apoptosis in the target cells by activation of caspases. Moreover, granzyme B was reported to cleave directly α-tubulin, leading to perturbation of microtubule networks during the induced cell death.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Kummer JA, Kamp AM, Tadema TM, Vos W, Meijer CJ, Hack CE: Localization and identification of granzymes A and B‑expressing cells in normal human lymphoid tissue and peripheral blood. Clin·Exp·Immunol. 1995·Apr; 100(1):164‑72. <·PMID:·7697916·>
• Kummer JA, Wever PC, Kamp AM, ten Berge IJ, Hack CE, Weening JJ: Expression of granzyme A and B proteins by cytotoxic lymphocytes involved in acute renal allograft rejection. Kidney·Int. 1995·Jan; 47(1):70‑7. <·PMID:·7731173·>
• Martin P, Pardo J, Schill N, Joeckel L, Berg M, Froelich CJ, Wallich R, Simon MM: Granzyme B‑induced and caspase 3‑dependent cleavage of gelsolin by mouse cytotoxic T cells modifies cytoskeleton dynamics. J·Biol·Chem. 2010·Apr·15; <·PMID:·23510371·>
• Rissoan MC, Duhen T, Bridon JM, Bendriss-Vermare N, Péronne C, de Saint Vis B, Brière F, Bates EE: Subtractive hybridization reveals the expression of immunoglobulin‑like transcript 7, Eph‑B1, granzyme B, and 3 novel transcripts in human plasmacytoid dendritic cells. Blood. 2002·Nov·1; 100(9):3295‑303. <·PMID:·12384430·>
• Spaeny-Dekking EH, Hanna WL, Wolbink AM, Wever PC, Kummer JA, Swaak AJ, Middeldorp JM, Huisman HG, Froelich CJ, Hack CE: Extracellular granzymes A and B in humans: detection of native species during CTL responses in vitro and in vivo. J·Immunol. 1998·Apr·1; 160(7):3610‑6. <·PMID:·9531325·>
• Sun Y, Schmitz JE, Acierno PM, Santra S, Subbramanian RA, Barouch DH, Gorgone DA, Lifton MA, Beaudry KR, Manson K, Philippon V, Xu L, Maecker HT, Mascola JR, Panicali D, Nabel GJ, Letvin NL: Dysfunction of simian immunodeficiency virus/simian human immunodeficiency virus‑induced IL‑2 expression by central memory CD4+ T lymphocytes. J·Immunol. 2005·Apr·15; 174(8):4753‑60. <·PMID:·15814700·>
• Tak PP, Spaeny-Dekking L, Kraan MC, Breedveld FC, Froelich CJ, Hack CE: The levels of soluble granzyme A and B are elevated in plasma and synovial fluid of patients with rheumatoid arthritis (RA). Clin·Exp·Immunol. 1999·May; 116(2):366‑70. <·PMID:·10337032·>