特異性

The mouse monoclonal antibody HD30 recognizes CD205 antigen, a 200 kDa C-type lectin transmembrane protein of the MMR (macrophage mannose receptor) family, expressed at high levels on dendritic cells and thymic epithelial cells, and at low levels on lymphocytes, NK cells and monocytes.

抗原情報

CD205 (DEC-205) is an endocytic receptor of macrophage mannose receptor family. This 205 kDa C-type lectin transmembrane protein mediates adsorptive uptake and its intracellular domain contains coated pit localization sequence and distal acidic motif, which is required for recycling beyond early endosomes through deeper MHC II+ late endosomes and lysosomes. This unique pathway of receptor-mediated uptake proves to be necessary for presentation of antigenic peptides at low doses of ligand. CD205 is responsible for uptake and processing of captured antigens for dendritic cells.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Guo M, Gong S, Maric S, Misulovin Z, Pack M, Mahnke K, Nussenzweig MC, Steinman RM: A monoclonal antibody to the DEC‑205 endocytosis receptor on human dendritic cells. Hum·Immunol. 2000·Aug; 61(8):729‑38. <·PMID:·10980384·>
• Gurer C, Strowig T, Brilot F, Pack M, Trumpfheller C, Arrey F, Park CG, Steinman RM, Münz C: Targeting the nuclear antigen 1 of Epstein‑Barr virus to the human endocytic receptor DEC‑205 stimulates protective T‑cell responses. Blood. 2008·Aug·15; 112(4):1231‑9. <·PMID:·18519810·>
• Mahnke K, Guo M, Lee S, Sepulveda H, Swain SL, Nussenzweig M, Steinman RM: The dendritic cell receptor for endocytosis, DEC‑205, can recycle and enhance antigen presentation via major histocompatibility complex class II‑positive lysosomal compartments. J·Cell·Biol. 2000·Oct·30; 151(3):673‑84. <·PMID:·11062267·>
• Park CG, Rodriguez A, Ueta H, Lee H, Pack M, Matsuno K, Steinman RM: Generation of anti‑human DEC205/CD205 monoclonal antibodies that recognize epitopes conserved in different mammals. J·immunol·Methods. 2012·Mar·30; 377(1‑2):15‑22. <·PMID:·22273672·>