特異性

The mouse monoclonal antibody UW40 recognizes GRAP2 (GADS), a 41 kDa cytoplasmic adaptor protein that plays a pivotal role during the early events of signal transduction in T cells.

抗原情報

GRAP2; Grb2-related adaptor protein 2 or GADS; Grb2-related adaptor downstream of Shc, is a cytoplasmic adaptor protein containing N- and C-terminal SH3 domains flanking a central SH2 domain and a proline/glutamine-rich region. It is expressed predominantly in lymphoid tissue and hematopoietic cells, particularly in T cells. GRAP2 (GADS) plays a pivotal role during the early events of T cell signal transduction by recruiting the adaptor protein SLP-76 and its associated molecules, such as Vav, Nck, Itk, and ADAP, to the transmembrane adaptor protein LAT. GRAP2 (GADS) also binds several other signaling proteins, namely Gab2, HPK1 (hematopoietic progenitor kinase 1), and Cbl. Unlike similar adaptor protein Grb2, GRAP2 (GADS) shows higher selectivity when binding to the particular phosphorylated tyrosines of LAT adaptor.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Cho S, Velikovsky CA, Swaminathan CP, Houtman JC, Samelson LE, Mariuzza RA: Structural basis for differential recognition of tyrosine‑phosphorylated sites inthe linker for activation of T cells (LAT) by the adaptor Gads. EMBO·J. 2004·Apr·7; 23(7):1441‑51. <·PMID:·15029250·>
• Dalheimer SL, Zeng L, Draves KE, Hassaballa A, Jiwa NN, Parrish TD, Clark EA, Yankee TM: Gads‑deficient thymocytes are blocked at the transitional single positive CD4+stage. Eur·J·Immunol. 2009·May; 39(5):1395‑404. <·PMID:·19337995·>
• Moran O, Roessle MW, Mariuzza RA, Dimasi N: Structural features of the full‑length adaptor protein GADS in solution determined using small‑angle X‑ray scattering. Biophys·J. 2008·Mar·1; 94(5):1766‑72. <·PMID:·17993503·>
• Watanabe R, Harada Y, Takeda K, Takahashi J, Ohnuki K, Ogawa S, Ohgai D, Kaibara N, Koiwai O, Tanabe K, Toma H, Sugamura K, Abe R: Grb2 and Gads exhibit different interactions with CD28 and play distinct roles in CD28‑mediated costimulation. J·Immunol. 2006·Jul·15; 177(2):1085‑91. <·PMID:·16818765·>
• Yankee TM, Draves KE, Ewings MK, Clark EA, Graves JD: CD95/Fas induces cleavage of the GrpL/Gads adaptor and desensitization of antigen receptor signaling. Proc·Natl·Acad·Sci·USA. 2001·Jun·5; 98(12):6789‑93. <·PMID:·11391000·>
• Yankee TM, Yun TJ, Draves KE, Ganesh K, Bevan MJ, Murali-Krishna K, Clark EA: The Gads (GrpL) adaptor protein regulates T cell homeostasis. J·Immunol. 2004·Aug·1; 173(3):1711‑20. <·PMID:·15265900·>