シスメックス株式会社

研究用フローサイトと理化学分析

研究用フローサイトと理化学分析

CyFlow TCR alpha/beta PE (RUO)

品番 CG376244
抗体名 Anti-Rt TCR ALPHA/BETA PE,R73
包装単位 0.1 mg
濃度 0.5 mg/ml
推奨使用量 -
容量 0.2 ml
関連製品
(アイソタイプコントロール)
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反応性|交差吸着 Rat | Non-Human Primates レーザー Blue, Green, Yellow
抗原 TCR α/β 最大蛍光波長 576 nm
クローン R73 最大励起波長 496 nm, 565 nm
ホスト Mouse 標識/Format PE
アイソタイプ IgG1 研究分野 Immunophenotyping | MHC
クローナリティ monoclonal アプリケーション

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Anti-Rt TCR ALPHA/BETA PE,R73

特異性

The mouse monoclonal R73 recognizes TCR α/β antigen, the dominant subtype of T cell receptor expressed in peripheral blood.

抗原情報

The antigen-specific T cell receptor (TCR) is composed of either α and β subunit, or γ and δ subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR α/β heterodimers, whereas the T cells expressing TCR γ/δ heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.

利用方法

The reagent is designed for Flow Cytometry analysis. Working concentrations should be determined by the investigator.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

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• Fuller JM, Bogdani M, Tupling TD, Jensen RA, Pefley R, Manavi S, Cort L, Blankenhorn EP, Mordes JP, Lernmark A, Kwitek AE: Genetic dissection reveals diabetes loci proximal to the gimap5 lymphopenia gene. Physiol·Genomics. 2009·Jun·10; 38(1):89‑97. <·PMID:·19351909·>
• Hansson AS, Heinegard D, Holmdahl R: A new animal model for relapsing polychondritis, induced by cartilage matrix protein (matrilin‑1). J·Clin·Invest. 1999·Sep; 104(5):589‑98. <·PMID:·10487773·>
• Hünig T, Wallny HJ, Hartley JK, Lawetzky A, Tiefenthaler G: A monoclonal antibody to a constant determinant of the rat T cell antigen receptor that induces T cell activation: Differential reactivity with subsets of immature and mature T lymphocytes. J·Exp·Med. 1989·Jan·1; 169(1):73‑86. <·PMID:·2783336·>
• Kerstan A, Hünig T: Cutting edge: distinct TCR‑ and CD28‑derived signals regulate CD95L, Bcl‑xL, and the survival of primary T cells. J·Immunol. 2004·Feb·1; 172(3):1341‑5. <·PMID:·14734708·>
• Kraus E, Lambracht D, Wonigeit K, Hünig T: Negative regulation of rat natural killer cell activity by major histocompatibility complex class I recognition. Eur·J·Immunol. 1996·Nov; 26(11):2582‑6. <·PMID:·8921942·>
• Kreiss M, Asmuss A, Krejci K, Lindemann D, Miyoshi-Akiyama T, Uchiyama T, Rink L, Broeren CP, Herrmann T: Contrasting contributions of complementarity‑determining region 2 and hypervariable region 4 of rat BV8S2+ (Vbeta8.2) TCR to the recognition of myelin basic protein and different types of bacterial superantigens. Int·Immunol. 2004·May; 16(5):655‑63. <·PMID:·15096488·>
• Li S, Thanikachalam M, Pang M, Kawaharada N, Aitouche A, Pham SM: A clinically relevant CTLA4‑Ig‑based regimen induces chimerism and tolerance to heart grafts. Ann·Thorac·Surg. 2001·Oct; 72(4):1306‑10. <·PMID:·11603451·>
• Müller N, van den Brandt J, Odoardi F, Tischner D, Herath J, Flügel A, Reichardt HM: A CD28 superagonistic antibody elicits 2 functionally distinct waves of T cell activation in rats. J·Clin·Invest. 2008·Apr; 118(4):1405‑16. <·PMID:·18357346·>
• Radojevic K, Arsenovic-Ranin N, Kosec D, Pesic V, Pilipovic I, Perisic M, Plecas-Solarovic B, Leposavic G: Neonatal castration affects intrathymic kinetics of T‑cell differentiation and the spleen T‑cell level. J·Endocrinol. 2007·Mar; 192(3):669‑82. <·PMID:·17332534·>
• Ritter T, Schröder G, Risch K, Vergopoulos A, Shean MK, Kolls J, Brock J, Lehmann M, Volk HD: Ischemia/reperfusion injury‑mediated down‑regulation of adenovirus‑mediated gene expression in a rat heart transplantation model is inhibited by co‑application of a TNFRp55‑Ig chimeric construct. Gene·Ther. 2000·Jul; 7(14):1238‑43. <·PMID:·10918493·>
• Shao H, Sun SL, Kaplan HJ, Sun D: Characterization of rat CD8+ uveitogenic T cells specific for interphotoreceptor retinal‑binding protein 1177‑1191. J·Immunol. 2004·Aug·15; 173(4):2849‑54. <·PMID:·15295004·>
• Wilhelm MJ, Pratschke J, Beato F, Taal M, Kusaka M, Hancock WW, Tilney NL: Activation of the heart by donor brain death accelerates acute rejection after transplantation. Circulation. 2000·Nov·7; 102(19):2426‑33. <·PMID:·11067799·>

Available Safety Data Sheets

CyFlow™ 抗体SDS(日本語)

Available Technical Data Sheets

CyFlow™ TCR alpha-beta PE (R73) CG376244 TDS (US)