特異性

The armenian hamster monoclonal antibody 22F6 recognizes Helios antigen, a transcription factor expressed in some hematopoietic stem cells, and at high levels in thymic-derived regulatory T cells.

抗原情報

Helios (IKZF2; Ikaros family zinc finger protein 2) is a hematopoietic-specific transcription factor involved in the regulation of lymphocyte development, together with other members of this family, such as Aiolos and Ikaros. Helios forms homo- and heterodimers with these proteins and is thought to function predominantly in early hematopoietic development. Expression of Helios, Aiolos and Ikaros is restricted to cells of the hematopoietic system, whereas other family members, Eos and Pegassus, are more widely expressed. Helios is expressed at early stages of thymocyte development. In mature T cells, Helios has been strongly associated with Treg cells.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Atarashi K, Tanoue T, Oshima K, Suda W, Nagano Y, Nishikawa H, Fukuda S, Saito T, Narushima S, Hase K, Kim S, Fritz JV, Wilmes P, Ueha S, Matsushima K, Ohno H, Olle B, Sakaguchi S, Taniguchi T, Morita H, Hattori M, Honda K: Treg induction by a rationally selected mixture of Clostridiastrains from the human microbiota. Nature. 2013·Aug·8; 500(7461):232‑6. <·PMID:·23842501·>
• Blankenhaus B, Reitz M, Brenz Y, Eschbach ML, Hartmann W, Haben I, Sparwasser T, Huehn J, Kühl A, Feyerabend TB, Rodewald HR, Breloer M: Foxp3+ regulatory T cells delay expulsion of intestinal nematodes by suppression of IL‑9‑driven mast cell activation in BALB/c but not in C57BL/6 mice. PLoS·Pathog. 2014·Feb·6; 10(2):e1003913. <·PMID:·24516385·>
• Daley SR, Hu DY, Goodnow CC: Helios marks strongly autoreactive CD4+ T cells in two major waves of thymic deletion distinguished by induction of PD‑1 or NF‑&#954B. J·Exp·Med. 2013·Feb·11; 210(2):269‑85. <·PMID:·23337809·>
• McIver Z, Melenhorst JJ, Wu C, Grim A, Ito S, Cho I, Hensel N, Battiwalla M, Barrett AJ: Donor lymphocyte count and thymic activity predict lymphocyte recovery and outcomes after matched‑sibling hematopoietic stem cell transplant. Haematologica. 2013·Mar; 98(3):346‑52. <·PMID:·23065508·>
• Pinheiro D, Singh Y, Grant CR, Appleton RC, Sacchini F, Walker KR, Chadbourne AH, Palmer CA, Armitage-Chan E, Thompson I, Williamson L, Cunningham F, Garden OA: Phenotypic and functional characterization of a CD4(+) CD25(high) FOXP3(high) regulatory T‑cell population in the dog. Immunology. 2011·Jan; 132(1):111‑22. <·PMID:·20880379·>
• Serre K, Bénézech C, Desanti G, Bobat S, Toellner KM, Bird R, Chan S, Kastner P, Cunningham AF, Maclennan IC, Mohr E: Helios is associated with CD4 T cells differentiating to T helper 2 and follicular helper T cells in vivo independently of Foxp3 expression. PLoS·One. 2011; 6(6):e20731. <·PMID:·25877073·>
• Thornton AM, Korty PE, Tran DQ, Wohlfert EA, Murray PE, Belkaid Y, Shevach EM: Expression of Helios, an Ikaros transcription factor family member, differentiates thymic‑derived from peripherally induced Foxp3+ T regulatory cells. J·Immunol. 2010·Apr·1; 184(7):3433‑41. <·PMID:·20181882·>