CyFlow CD99R PE (RUO)
| 品番 | CR938750 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD99R PE,MEM-131 | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 推奨使用量 | - | ||
| 容量 | 2 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgM PE (CW330883) |
||
| 反応性|交差吸着 | Human | レーザー | Blue, Green, Yellow |
| 抗原 | CD99R, E2 | 最大蛍光波長 | 576 nm |
| クローン | MEM-131 | 最大励起波長 | 496 nm, 565 nm |
| ホスト | Mouse | 標識/Format | PE |
| アイソタイプ | IgM | 研究分野 | Immunophenotyping |
| クローナリティ | monoclonal | アプリケーション | - |
Anti-Hu CD99R PE,MEM-131
特異性
The mouse monoclonal antibody MEM-131 recognizes CD99R antigen, an epitope restricted to a subset of CD99 molecule expressed on myeloid cells, NK cells and T lymphocytes.
抗原情報
CD99 (E2, MIC2) is a transmembrane glycoprotein that is involved in regulation of T cell addhesive properties and programmed cell death distinct from typical apoptosis course. CD99 roles are specific to certain stages of T cell differentiation such as corticothymocytes. CD99R isoform expression is restricted in the haematopoietic system to T, NK and myeloid cells.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Bernard G, Breittmayer JP, de Matteis M, Trampont P, Hofman P, Senik A, Bernard A: Apoptosis of immature thymocytes mediated by E2/CD99. J·Immunol. 1997·Mar·15; 158(6):2543‑50. <·PMID:·9058785·>
• Bernard G, Raimondi V, Alberti I, Pourtein M, Widjenes J, Ticchioni M, Bernard A: CD99 (E2) up‑regulates alpha4beta1‑dependent T cell adhesion to inflamed vascular endothelium under flow conditions. Eur·J·Immunol. 2000·Oct; 30(10):3061‑5. <·PMID:·11069091·>
• Bernard G, Zoccola D, Deckert M, Breittmayer JP, Aussel C, Bernard A: The E2 molecule (CD99) specifically triggers homotypic aggregation of CD4+ CD8+thymocytes. J·Immunol. 1995·Jan·1; 154(1):26‑32. <·PMID:·7527813·>
• Cermák L, Símová S, Pintzas A, Horejsí V, Andera L: Molecular mechanisms involved in CD43‑mediated apoptosis of TF‑1 cells: Roles of transcription Daxx expression, and adhesion molecules. J·Biol·Chem. 2002·Mar·8; 277(10):7955‑61. <·PMID:·11773067·>
• Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·>
• Gelin C, Aubrit F, Phalipon A, Raynal B, Cole S, Kaczorek M, Bernard A: The E2 antigen, a 32 kd glycoprotein involved in T‑cell adhesion processes, isthe MIC2 gene product. EMBO·J. 1989·Nov; 8(11):3253‑9. <·PMID:·2479542·>
• Olweus J, Lund-Johansen F, Terstappen LW: CD64/Fc gamma RI is a granulo‑monocytic lineage marker on CD34+ hematopoietic progenitor cells. Blood. 1995·May·1; 85(9):2402‑13. <·PMID:·7537112·>