CyFlow CD45R0 PE-Cy7 (RUO)
| 品番 | CS876696 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD45R0 PE-CY7,UCHL1 | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 推奨使用量 | - | ||
| 容量 | 0.4 ml | ||
| 関連製品 (アイソタイプコントロール) |
- | ||
| 反応性|交差吸着 | Human | レーザー | Blue, Green, Yellow |
| 抗原 | CD45R0, UCHL-1 | 最大蛍光波長 | 785 nm |
| クローン | UCHL1 | 最大励起波長 | 496 nm, 565 nm |
| ホスト | Mouse | 標識/Format | PE-Cy7 |
| アイソタイプ | IgG2a | 研究分野 | Immunophenotyping | Phosphorylation |
| クローナリティ | monoclonal | アプリケーション | - |
Anti-Hu CD45R0 PE-CY7,UCHL1
特異性
The mouse monoclonal antibody UCHL1 recognizes CD45R0, a 180 kDa low molecular weight isoform of the leukocyte common antigen (LCA). CD45R0 is expressed on a subset of memory/activated T cells and on cortical thymocytes.
抗原情報
CD45R0 is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45R0 is expressed e.g. on macrophages, CD8+ T cells, activated T cells and myeloma cells.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Akbar AN, Terry L, Timms A, Beverley PC, Janossy G: Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T cells. J·Immunol. 1988·Apr·1; 140(7):2171‑8. <·PMID:·2965180·>
• Beverley PC: Human T cell subsets. Immunol·Lett. 1987·Apr; 14(4):263‑7. <·PMID:·2953677·>
• Beverley PC, Merkenschlager M, Terry L: Phenotypic diversity of the CD45 antigen and its relationship to function. Immunol·Suppl. 1988; 1:3‑5. <·PMID:·2968306·>
• Bijian K, Zhang L, Shen SH: Collagen‑mediated survival signaling is modulated by CD45 in Jurkat T cells. Mol·Immunol. 2007·Jul; 44(15):3682‑90. <·PMID:·17524482·>
• Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV‑1 encephalitis.. Brain·Pathol. 2006·Oct; 16(4):256‑65. <·PMID:·17107594·>
• Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ: Combinations of CD45 isoforms are crucial for immune function and disease. J·Immunol. 2006·Mar·15; 176(6):3417‑25. <·PMID:·16517710·>
• Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Apoptosis. 2008·Feb; 13(2):197‑212. <·PMID:·18157742·>
• Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·>
• Li FJ, Tsuyama N, Ishikawa H, Obata M, Abroun S, Liu S, Otsuyama K, Zheng X, Ma Z, Maki Y, Kawano MM: A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL‑6‑induced proliferation in myeloma. Blood. 2005·Apr·15; 105(8):3295‑302. <·PMID:·15626731·>
• Merkenschlager M, Terry L, Edwards R, Beverley PC: Limiting dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T cell memory formation. Eur·J·Immunol. 1988·Nov; 18(11):1653‑61. <·PMID:·2974420·>
• Norton AJ, Ramsay AD, Smith SH, Beverley PC, Isaacson PG: Monoclonal antibody (UCHL{01} that recognises normal and neoplastic T cells in routinely fixed tissues. J·Clin·Pathol. 1986·Apr; 39(4):399‐405. <·PMID:·2422219·>
• Smith SH, Brown MH, Rowe D, Callard RE, Beverley PC: Functional subsets of human helper‑inducer cells defined by a new monoclonal antibody, UCHL1. Immunology. 1986·May; 58(1):63‑70. <·PMID:·2423439·>
• Terry LA, Brown MH, Beverley PC: The monoclonal antibody, UCHL1, recognizes a 180,000 MW component of the human leucocyte‑common antigen, CD45. Immunology. 1988·Jun; 64(2):331‑6. <·PMID:·2455685·>
• McMichael AJ, Beverley PCL, Cobbold S, et al (Eds): Leucocyte Typing III, White Cell Differentiation Antigens. Oxford·University·Press,·Oxford. 1987; 1‑1050. <·NLM·ID:·8913266·>
• Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford·University·Press,·Oxford. 1989; 1‑1820. <·NLM·ID:·8914679·>