CyFlow CD314 PE (RUO)
| 品番 | CT402652 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD314 PE,1D11 | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 推奨使用量 | - | ||
| 容量 | 1 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG1 PE (BV655324) |
||
| 反応性|交差吸着 | Human | レーザー | Blue, Green, Yellow |
| 抗原 | CD314, NKG2D | 最大蛍光波長 | 576 nm |
| クローン | 1D11 | 最大励起波長 | 496 nm, 565 nm |
| ホスト | Mouse | 標識/Format | PE |
| アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
| クローナリティ | monoclonal | アプリケーション | - |
Anti-Hu CD314 PE,1D11
特異性
The mouse monoclonal antibody 1D11 recognizes CD314 antigen, a 42 kDa C-type lectin-like activating receptor expressed by NK cells, γ/δ T cells, and CD8+ T cells.
抗原情報
CD314 (NKG2D; natural killer receptor G2D or KLRK1; killer cell lectin-like receptor subfamily K, member 1), is a homodimeric C-type lectin-like activating receptor and costimulator with type II membrane orientation (C teminus extracellular). CD314 homodimers are associated with DAP10, a membrane adaptor protein that signals similar to CD28 by recruitment of phosphatidylinositol 3-kinase. Engagement of CD314 amplifies antigen-specific T cell responses in CD314-positive T cell populations. In NK cells, CD314 is a primary activating receptor. As CD314 ligands the MHC class-I chain-related proteins A and B (MICA, MICB) and UL16-binding proteins (ULBPs) have been identified.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Bauer S, Groh V, Wu J, Steinle A, Phillips JH, Lanier LL, Spies T: Activation of NK cells and T cells by NKG2D, a receptor for stress‑inducible MICA. Science. 1999·Jul·30; 285(5428):727‑9. <·PMID:·10426993·>
• Ebert LM, Meuter S, Moser B: Homing and function of human skin gammadelta T cells and NK cells: relevance for tumor surveillance. J·Immunol. 2006·Apr·1; 176(7):4331‑6. <·PMID:·16547270·>
• Hasenkamp J, Borgerding A, Uhrberg M, Falk C, Chapuy B, Wulf G, Jung W, Trümper L, Glass B: Self‑tolerance of human natural killer cells lacking self‑HLA‑specific inhibitoryreceptors. Scand·J·Immunol. 2008·Mar; 67(3):218‑29. <·PMID:·18226015·>
• Sangiolo D, Martinuzzi E, Todorovic M, Vitaggio K, Vallario A, Jordaney N, Carnevale-Schianca F, Capaldi A, Geuna M, Casorzo L, Nash RA, Aglietta M, Cignetti A: Alloreactivity and anti‑tumor activity segregate within two distinct subsets of cytokine‑induced killer (CIK) cells: implications for their infusion across major HLA barriers. Int·Immunol. 2008·Jul; 20(7):841‑8. <·PMID:·18469328·>
• Valencia J, Hernández-López C, Martínez VG, Hidalgo L, Zapata AG, Vicente A, Varas A, Sacedón R: Transient beta‑catenin stabilization modifies lineage output from human thymic CD34+CD1a‑ progenitors. J·Leukoc·Biol. 2010·Mar; 87(3):405‑14. <·PMID:·19952356·>
• Wu J, Cherwinski H, Spies T, Phillips JH, Lanier LL: DAP10 and DAP12 form distinct, but functionally cooperative, receptor complexes in natural killer cells. J·Exp·Med. 2000·Oct·2; 192(7):1059‑68. <·PMID:·11015446·>
• Wu J, Groh V, Spies T: T cell antigen receptor engagement and specificity in the recognition of stress‑inducible MHC class I‑related chains by human epithelial gamma delta T cells. J·Immunol. 2002·Aug·1; 169(3):1236‑40. <·PMID:·12133944·>