特異性

The mouse monoclonal antibody 87G recognizes both membrane-bound and soluble forms of HLA-G (HLA-G1 and HLA-G5). HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells. The antibody 87G blocks interaction of HLA-G with inhibitory receptors.

抗原情報

利用方法

The reagent is designed for Flow Cytometry analysis. Suggested working usage is 10 µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Hackmon R, Hallak M, Krup M, Weitzman D, Sheiner E, Kaplan B, Weinstein Y: HLA‑G antigen and parturition: maternal serum, fetal serum and amniotic fluid levels during pregnancy. Fetal·Diagn·Ther. 2004·Sep-Oct; 19(5):404‑9. <·PMID:·15305096·>
• Ishitani A, Sageshima N, Lee N, Dorofeeva N, Hatake K, Marquardt H, Geraghty DE: Protein Expression and Peptide Binding Suggest Unique and Interacting Functional Roles for HLA‑E, F and G in Maternal‑Placental Immune Recognitions. J·Immunol. 2003; 171:1376. <·PMID:·12874228·>
• Lee N, Malacko AR, Ishitani A, Chen MC, Bajorath J, Marquardt H, Geraghty DE: The membrane‑bound and soluble forms of HLA‑G bind identical sets of endogenous peptides but differ with respect to TAP association. Immunity. 1995; 3(5):591‑600. <·PMID:·7584149·>
• LeMaoult J, Caumartin J, Daouya M, Favier B, Le Rond S, Gonzalez A, Carosella ED: Immune regulation by pretenders: cell‑to‑cell transfers of HLA‑G make effector T cells act as regulatory cells. Blood. 2007·Mar·1; 109(5):2040‑8. <·PMID:·17077329·>
• Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N: Characterization of monoclonal antibodies recognizing HLA‑G or HLA‑E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum·Immunol. 2003·Mar; 64(3):315‑26. <·PMID:·12590976·>
• Polakova K, Bandzuchova E, Hofmeister V, Weiss EH, Hutter H, Russ G: Binding analysis of HLA‑G specific antibodies to hematopoietic cells isolated from leukemia patients. Neoplasma. 2003; 50(5):331‑8. <·PMID:·14628085·>
• Polakova K, Krcova M, Kuba D, Russ G: Analysis of HLA‑G expression in malignant hematopoetic cells from leukemia patients. Leuk·Res. 2003·Jul; 27(7):643‑8. <·PMID:·12681364·>
• Riteau B, Menier C, Khalil-Daher I, Martinozzi S, Pla M, Dausset J, Carosella ED, Rouas-Freiss N: HLA‑G1 co‑expression boosts the HLA class I‑mediated NK lysis inhibition. Int·Immunol. 2001·Feb; 13(2):193‑201. <·PMID:·11157852·>
• Rouas-Freiss N, Moreau P, Ferrone S, Carosella ED: HLA‑G proteins in cancer: do they provide tumor cells with an escape mechanism?. Cancer·Res. 2005·Nov·15; 65(22):10139‑44. <·PMID:·16287995·>
• Sageshima N, Ishitani A, Omura M, Akasaki M, Umekage H, Katabuchi H, Okamura H, Hatake K: Necrotic feature of the trophoblasts lacking HLA‑G expression in normal andpre‑eclamptic placentas. Am·J·Reprod·Immunol. 2003·Mar; 49(3):174‑82. <·PMID:·12797524·>
• Shobu T, Sageshima N, Tokui H, Omura M, Saito K, Nagatsuka Y, Nakanishi M, Hayashi Y, Hatake K, Ishitani A: The surface expression of HLA‑F on decidual trophoblasts increases from mid to term gestation. J·Reprod·Immunol. 2006·Dec; 72(1‑2):18‑32. <·PMID:·16806485·>
• Wiendl H: The non‑classical MHC molecule HLA‑G protects human muscle cells from immune‑mediated lysis: implications for myoblast transplantation and gene therapy. Brain. 2003; 126(1):176‑85. <·PMID:·12477705·>