CyFlow IgE PE (RUO)
| 品番 | CX012682 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu IGE PE,BE5 | ||
| 包装単位 | 0.1 mg | ||
| 濃度 | 0.1 mg/ml | ||
| 推奨使用量 | - | ||
| 容量 | 1 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG1 PE (BV655324) |
||
| 反応性|交差吸着 | Human | レーザー | Blue, Green, Yellow |
| 抗原 | IgE | 最大蛍光波長 | 576 nm |
| クローン | BE5 | 最大励起波長 | 496 nm, 565 nm |
| ホスト | Mouse | 標識/Format | PE |
| アイソタイプ | IgG1 | 研究分野 | Immunoglobulins |
| クローナリティ | monoclonal | アプリケーション | - |
Anti-Hu IGE PE,BE5
特異性
The mouse monoclonal antibody BE5 recognizes human immunoglobulin E (IgE); it recognizes an epitope different from the ones recognized by 4G7 and 4H10 antibodies to IgE.
抗原情報
Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.
利用方法
The reagent is designed for Flow Cytometry analysis. Suggested working usage is 5 µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Balogh Z, Merétey K, Falus A, Bozsóky S: Serological abnormalities in juvenile chronic arthritis: a review of 46 cases. Ann·Rheum·Dis. 1980·Apr; 39(2):129‑34. <·PMID:·6966908·>
• Brinkmann V, Heusser CH: T cell‑dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell·Immunol. 1993·Dec; 152(2):323‑32. <·PMID:·8258141·>
• Franklin EC: Structure and function of immunoglobulins. Acta·Endocrinol·Suppl·(Copenh). 1975; 194:77‑95. <·PMID:·47690·>
• Fuller JM, Keyser JW: Serum immunoglobulins after surgical operation. Clin·Chem. 1975·May; 21(6):667‑71. <·PMID:·1122610·>
• Gould HJ, Beavil RL, Vercelli D: IgE isotype determination: epsilon‑germline gene transcription, DNA recombination and B‑cell differentiation. Br·Med·Bull. 2000; 56(4):908‑24. <·PMID:·11359628·>
• Kaulfürst-Soboll H, Mertens M, Brehler R, von Schaewen A: Reduction of cross‑reactive carbohydrate determinants in plant foodstuff: elucidation of clinical relevance and implications for allergy diagnosis. PLoS·One. 2011·Mar·14; 6(3):e17800. <·PMID:·21423762·>