特異性

The mouse monoclonal antibody MEM-68 recognizes CD17 antigen, a membrane lipid moiety lactosylceramide expressed on granulocytes, monocytes and platelets.

抗原情報

CD17 (lactosylceramide) is an ubiquitous glycosphingolipid with uncharged disaccharide headgroup, highly enriched in lipid raft-derived structures. Besides playing a pivotal role in the biosynthesis of complex glycosphingilipids, lactosylceramide is involved in cell-cell and cell-matrix interactions and in signaling events linked to cell differentiation, development, apoptosis and oncogenesis. Lactosylceramide regulates integrin functions and production of nitric oxide. Its expression defines successive stages in the maturation of myeloid cells.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Bhunia AK, Arai T, Bulkley G, Chatterjee S: Lactosylceramide mediates tumor necrosis factor‑alpha‑induced intercellular adhesion molecule‑1 (ICAM‑1 expression and the adhesion of neutrophil in human umbilical vein endothelial cells. J·Biol·Chem. 1998·Dec·18; 273(51):34349‑57. <·PMID:·9852101·>
• Pannu R, Singh AK, Singh I: A novel role of lactosylceramide in the regulation of tumor necrosis factor alpha‑mediated proliferation of rat primary astrocytes: Implications for astrogliosis following neurotrauma. J·Biol·Chem. 2005·Apr·8; 280(14):13742‑51. <·PMID:·15668227·>
• Sharma DK, Brown JC, Cheng Z, Holicky EL, Marks DL, Pagano RE: The glycosphingolipid, lactosylceramide, regulates beta1‑integrin clustering and endocytosis. Cancer·Res. 2005·Sep·15; 65(18):8233‑41. <·PMID:·16166299·>
• Spychalska J, Smoleńska-Sym G, Zdebska E, Woźniak J, Kościelak J: Quantitative analysis of LacCer/CDw17 in human myelogenous leukaemic cells. Cell·Mol·Biol·Lett. 2003; 8(4):911‑7. <·PMID:·14668914·>
• Zhai X, Li XM, Momsen MM, Brockman HL, Brown RE: Lactosylceramide: lateral interactions with cholesterol. Biophys·J. 2006·Oct·1; 91(7):2490‑500. <·PMID:·16829567·>